• Introduction
• 3 min
  1. Editor's foreword

  • Prof. Ann Daly
• 47 min
  2. Introduction to drug metabolism enzymes

  • Dr. Dennis Smith
• General factors affecting drug metabolism
• 30 min
  3. General factors affecting drug metabolism: effect of physiological factors and disease 1

  • Prof. Edward T. Morgan
• 24 min
  4. General factors affecting drug metabolism: effect of physiological factors and disease 2

  • Prof. Edward T. Morgan
• 39 min
  5. Drug metabolism in liver disease

  • Dr. Nathalie Zgheib
  • Prof. Robert Branch
• 37 min
  6. Laboratory methods for the in vitro study of drug metabolism

  • Dr. Ritu Singh
• 50 min
  7. Prediction of pathways of drug metabolism

  • Dr. Maurice Dickins
• Phase I metabolizing enzymes: cytochrome P450s
• 45 min
  8. Cytochrome P450 1 family: the roles of 1A1, 1A2 and 1B1 in drug metabolism

  • Prof. F. Peter Guengerich
• 42 min
  9. CYP2 family

  • Prof. Ann Daly
• 52 min
  10. Why study the cytochrome P4503A (CYP3A) family?

  • Dr. Erin Schuetz
• 54 min
  11. Pharmacogenomics: an update

  • Prof. Magnus Ingelman-Sundberg
• Phase I metabolizing enzymes: non-cytochrome P450s
• 45 min
  12. Non-P450 oxidative metabolism: characteristics and drug substrates

  • Dr. Christine Beedham
• 53 min
  13. UDP-glucuronosyltransferases

  • Prof. Abby Collier
• Phase II metabolizing enzymes: conjugating enzymes
• 31 min
  14. Glutathione transferases

  • Prof. Ralf Morgenstern
• 31 min
  15. Arylamine N-acetyltransferases 1

  • Prof. Edith Sim
• 23 min
  16. Arylamine N-acetyltransferases 2

  • Prof. Edith Sim
• 19 min
  17. Arylamine N-acetyltransferases 3

  • Prof. Edith Sim
• 56 min
  18. Methyltransferases

  • Prof. Richard Weinshilboum
• 22 min
  19. Amino acid conjugation: mechanism and enzymology

  • Dr. Kathleen Knights
• Clinical aspects
• 33 min
  20. Clinical significance of enzyme induction and inhibition

  • Prof. Kim Brøsen
• 39 min
  21. Clinical importance of pharmacogenetic polymorphisms affecting drug metabolism

  • Prof. Julia Stingl (formerly Kirchheiner)
• Latest Updates in the Field
• 34 min
  22. Mammalian flavin-containing monooxygenases

  • Prof. Allan Rettie
• Archived Lectures *These may not cover the latest advances in the field
• 54 min
  23. Glucuronidation and the UDP - glucuronosyltransferases

  • Prof. Peter Mackenzie
  • Prof. John Miners
• 38 min
  24. General factors affecting drug metabolism: the role of nuclear receptor superfamily members in induction

  • Dr. Patrick Maurel
• 28 min
  25. Catalytic cycle of cytochrome P450s

  • Prof. Gordon Roberts
• 40 min
  26. Drug metabolism and liver disease

  • Prof. Robert Branch
• 46 min
  27. Crystal structures of drug-metabolizing P450 monooxygenases

  • Prof. Eric Johnson
• 49 min
  28. Sulfation and human cytosolic sulfotransferases

  • Prof. Charles Falany
• 50 min
  29. Laboratory methods for the in vitro study of drug metabolism

  • Dr. Charles Crespi
• 45 min
  30. Clinical importance of pharmacogenetic polymorphisms affecting drug metabolism: psychopharmacology and pain

  • Prof. Julia Stingl (formerly Kirchheiner)
• 51 min
  31. Genetic variability in Paraoxonase 1 (PON1), an HDL associated esterase: impact on risk and pharmacokinetics

  • Res. Prof. Clement Furlong
• 53 min
  32. Pharmacogenomics

  • Prof. Magnus Ingelman-Sundberg

Printable Handouts

PDF

Navigable Slide Index

1. P450s catalytic cycle
2. Reaction catalyzed by P450
3. Catalytic cycle - reviews
4. The catalytic cycle
5. Catalytic cycle - side reactions
6. Substrate binding
7. Substrate binding to CYP BM3
8. Substrate binding to CYP2C9
9. Oxygen cleavage & substrate hyd.
10. Catalytic cycle - electron transfer
11. Electron sources
12. P450 monooxygenase in the ER
13. NADPH-P450 reductase (CPR)
14. CPR - cofactors
15. CPR - kinetics & thermodynamics
16. Interflavin electron transfer - T-jump
17. Interflavin electron transfer - rates
18. Electron transfer viscosity effect
19. CPR - domain motion
20. Coenzyme effects on domain motion
21. Cytochrome b5
22. b5 and P450s
23. b5 - electron transfer
24. b5 - allosteric effects & summary
25. Acknowledgements

Topics Covered

• Catalytic cycle of cytochrome P450s
• Substrate and oxygen binding
• Key electron and proton transfers
• Dioxygen cleavage
• Formation of the 'activated oxygen' hydroxylating species
• Structure and electron transfer properties of NADPH-cytochrome P450 reductase
• Possible roles of cytochrome b5 in the p450 monooxygenase system

Links

Series:

• Drug Metabolizing Enzymes

Categories:

• Biochemistry
• Metabolism & Nutrition
• Pharmaceutical Sciences

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on August 14, 2015

Financial Disclosures

• Prof. Gordon Roberts has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.