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Printable Handouts
Navigable Slide Index
- Intro slide
- Introduction
- NF-kappa B is an ubiquitously expressed inducible TF
- Rel/NF-kappa B family of proteins
- Ikappa B family of proteins
- Structure of NF-kappa B p50:p65 bound to DNA
- Structure of the ankyrin-repeat domain of Ikappa B alpha
- Structure of NF-kappa B p50:p65 bound to Ikappa B alpha
- NF-kappa B activation: a wide variety of inducers
- NF-kappa B induce expression of many target genes
- NF-kappa B:responses to infection/stress/injury
- NF-kappa B as an effector in innate immune responses
- NF-kappa B as an effector in antigen receptor signaling
- NF-kappa B as an anti-apoptotic survival factor
- NF-kappa B protects cells from apoptosis
- Major biological roles of NF-kappa B
- NF-kappa B requires IkappaB modifications
- Ikappa B kinase (IKK) complex
- Classical NF-kappa B signaling pathway
- Analysis of KO mice: distinct roles of IKK subunits
- Alternative NF-kappa B signaling pathway
- The two alternative pathways to NF-kappa B activation
- Signaling pathways that lead to NF-kappa B activation
- Nuclear translocation/modification of NF-kappa B
- NF-kappa B modification: p65 phosphorylation
- p65 phosphorylation on serine 276 and PKA/MSK
- p65 phosphorylation: association with CBP/p300
- Nuclear NF-kappa B activity is regulated by HDACs
- Regulation of NF-kappa B by CBP and HDAC-1
- NF-kappa B activity dysregulation may lead to disease
- Diseases linked to dysregulation of NF-kappa B activity
- NF-kappa B inhibition: therapeutic importance
- Drugs and natural compounds as NF-kappa B inhibitors
- Specific inhibitors of the NF-kappa B pathway
- Advantages/disadvantages of blocking interactions
- NEMO binding domain or NBD
- NBD: hydrophobic finger in the C-terminus of IKKs
- Transport of NBD-peptide into cells
- Inhibition of NF-kappa B activation by the NBD-peptide
- Disrupting NEMO/NBD interactions: advantages
- Rapid and efficient uptake of NBD-peptide by cells
- Uptake/clearance of NBD in peripheral whole blood
- Can cell-permeable NBD-peptide work in animals?
- WT NBD-peptide ammeliorates acute inflammation
- Carrageennan-induced paw swelling in mice
- WT NBD-peptide inhibits NF-kappa B activation
- NBD-peptide administration: CIA as a model
- Intervention in CIA by NBD-peptide administration
- Inhibition of NF-kappa B by NBD-peptide inhibits CIA
- NBD-peptide inhibits paw thickness and arthritis
- NBD-peptide inhibits NF-kappa B DNA binding activity
- NBD-peptide inhibits cytokine production in CIA
- Efficacy of NBD-peptide treatment on CIA
- Inhibition of NF-kappa B by NBD-peptide appears safe
- Summary
Topics Covered
- NF-kappa B activation and function
- Signal transduction pathway
- Mechanism of post-transcriptional induction
- Gene activation
- Role of NF-kappa B in physiological processes
- Initiation of the immune system
- Cell survival
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Talk Citation
Ghosh, S. (2015, August 31). The transcription factor NF-Kappa B: an evolutionarily conserved mediator of immune and inflammatory responses [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 4, 2024, from https://doi.org/10.69645/RAXT9202.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Sankar Ghosh has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
The transcription factor NF-Kappa B: an evolutionarily conserved mediator of immune and inflammatory responses
A selection of talks on Immunology
Transcript
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0:00
SANKAR GHOSH:
The Transcription Factor NF-κB,
An Evolutionarily Conserved Mediator of
Immune and Inflammatory Responses,
presented by Sankar Ghosh,
professor, section of Immunobiology
and Department of Molecular
Biophysics & Biochemistry,
Yale University School of Medicine.
0:15
Inducible transcription factors play
a key role in communicating changes
in the environment
to the cell, thereby
allowing the cell to
adapt by modifying
its pattern of gene expression.
In mammalian cells, engagement
of cell surface receptors
trigger signal transduction pathways
that ultimately lead to activation
of certain transcription factors,
either through transcriptional
or post-transcriptional mechanisms.
Well-known examples of
inducible transcription factors
include steroid hormone receptors,
activating protein-1, or AP-1,
and of course, NF-κB.
0:49
NF-κB is an
ubiquitously expressed
inducible transcription factor.
It is found in all
mammalian cells and tissues.
However, it is present
in an inactive form.
It is inactive because it is bound
to a class of inhibitory proteins
known as IκBs, and the binding
of IκB masks the nuclear
localizing signal on
NF-κB, thereby
sequestering this NF-κB/IκB
complex in the cytoplasm.
When cells are stimulated, the
activation of signal transduction
pathways ultimately lead
to the phosphorylation
and degradation of IκB,
and we'll be talking
more about in subsequent slides.
That degraded IκB then
releases the bound NF-κB,
thereby unmasking the nuclear
localizing signals on NF-κB,
which then migrates into the
nucleus and binds to NF-κB
binding sites on promoters of
gene, thereby driving NF-κB
responsive gene expression.
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