Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Overview - RNA interference as an antiviral tool
3. RNA interference pathway
4. RNA interference methods
5. Antisense vs. RNAi
6. Inhibition of HIV-1 by RNAi
7. Virus inhibition by RNAi - RNA viruses
8. Virus inhibition by RNAi - DNA viruses
9. Coxsackievirus B3 (CVB-3)
10. Picornavirus replication
11. Dilatated cardiomyopathy (DCM)
12. Overview - design of efficient siRNAs
13. siRNAs targeting the Coxsackievirus 5' UTR
14. siRNAs against 5' UTR of CVB-3 have no effect
15. Screening for efficient siRNAs against TRPV1
16. What makes an efficient siRNA?
17. Efficient siRNA - strand bias or target structure?
18. Accessible nucleotides at 3' or 5' end of target site
19. Activation of seemingly inactive siRNA
20. Folding of artificial siRNA targets
21. siRNA silencing and local stability - correlation
22. Model for determinants of siRNA efficiency
23. Overview - inhibition of coxsackievirus replication
24. Targeting the viral RdRP
25. Coxsackievirus replication in cell culture
26. Cell viability assay with siRdRP 1-4
27. Inhibition of virus replication by siRNA
28. Inhibition of Coxsackievirus B3 by siRNA
29. CVB-3 inhibition in vivo
30. Overview - strand selectivity of virus silencing
31. Replication cycle of picornaviruses
32. Selective strand silencing
33. Strand-selectivity of siRdRP
34. Design of siRNAs targeting the RdRP (-)-strand
35. Strand-specificity of silencing
36. Strand specific siRNAs
37. Strand-inactivation by incorporation of LNA
38. siRNA vs. siLNA
39. Overview - SiDEx
40. Silencing of RdRP by shRNA expression plasmid
41. Long-term silencing generates escape mutants
42. Silencing of mutated RdRP
43. SiRNA double expression vector (SiDEx)
44. SiDEx silences two target sites
45. SiDEx silences wt and mutated RdRP
46. SiDEx - silencing of mutated RdRP
47. Silencing of mutated target site 4
48. Overview - silencing of CAR
49. Coxsackievirus-adenovirus-receptor (CAR)
50. Screening for efficient siRNAs targeting CAR
51. Efficiency of CAR-siRNAs
52. Duration of silencing
53. Inhibition of virus propagation by CAR-siRNA
54. SiDEx vector against two target sites of CVB-3
55. Summary
56. Acknowledgement

Topics Covered

• Coxsackievirus B3
• Design of efficient antiviral siRNAs
• Plaque reduction assays
• Virus escape
• siRNA double expression vectors (SiDEx)
• Strand selectivity of virus silencing
• Silencing of the host receptor (Coxsackievirus-adenovirus receptor)

Links

Series:

• RNA Interference

Categories:

• Cell Biology
• Genetics & Epigenetics
• Microbiology
• Pharmaceutical Sciences

Therapeutic Areas:

• Cardiovascular & Metabolic
• Infectious Diseases

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on April 17, 2016

Financial Disclosures

• Dr. Jens Kurreck has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.