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Hello, my name is Mel Bedi,
and today I'll be speaking to you on the subject of the creation of
tools for the investigation of oxidative damage to nucleic acids.
We'll begin this talk by providing
a brief introductory background on oxidative stress, and its implications.
We will then take a look at the approach that the Bryant-Friedrich group takes
in the creation of tools to study oxidative damage involving nucleic acids.
Finally, we'll discuss a novel method for reverse
automated RNA synthesis, employing H-phosphonate chemistry.
Let's begin with our introduction on oxidative stress.
Oxidative stress is one of the major contributors to the endogenous exposome.
It is a condition that arises when the proliferation of
reactive oxygen species (ROS) overwhelms the cell's ability to detoxify them.
This leads to damage to cellular components.
Sources of reactive oxygen species are both endogenous and exogenous.
Endogenously, reactive oxygen species can be
produced and released by the endoplasmic reticulum,
the mitochondria, the peroxisome, and the macrophages.
Some of the exogenous sources of reactive oxygen species include UV light from the sun,
ionizing radiation, and environmental pollutants.
At the cellular level, the proliferation of reactive oxygen species during
oxidative stress can cause damage to all the macromolecules,
including lipids, proteins, DNA, and RNA.