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1. Introduction
2. Receptor tyrosine kinases
3. Signal transduction pathways
4. PLC-gamma
5. PLC-gamma is an PIP2-specific hydrolytic enzyme
6. What is PIP2?
7. What is DAG?
8. Diacylglycerol production
9. What is IP3 ?
10. How is PLC-gamma activated?
11. The PI-specific PLC family
12. What defines a phospholipase C?
13. Domains in PLC-gamma 1
14. Association of PLC-gamma 1 with the receptor
15. PLC-gamma 1 association with the membrane
16. Tyrosine phosphorylation of PLC-gamma 1
17. PLC-gamma 1 Verses PLC-gamma 2
18. Measurement of PLC-gamma 1
19. Measurement of PLC-gamma tyrosine phosphorylation
20. In vivo measurement of IP3
21. In vivo measurement of intracellular Ca2+ release
22. Measurement of PLC-gamma 1 activity in vitro
23. T-Cell proliferation
24. Role of immediate early genes in cell proliferation
25. Downstream effects of PLC signaling
26. Role of PLC-gamma in IL-2 expression
27. T-cell receptor activation
28. TK phosphorylation of PLC-gamma 1 in T-cells
29. Calcium regulation of IL-2 transcription
30. DAG control of IL-2 transcription (1)
31. DAG control of IL-2 transcription (2)
32. Role of PLC-gamma in IL-2 expression
33. Cell morphogenesis
34. Role of integrins in cell morphogenesis
35. Role of PLC-gamma 1 in cell morphogenesis
36. PLC-gamma biologic roles

Topics Covered

• PLC-gamma in growth factor signaling
• PLC-gamma as an enzyme
• Substrate and products
• PLC family members
• Domains
• Association of PLC-gamma with activated receptor tyrosine kinases and membranes
• Tyrosine phosphorylation sites of PLC-gamma
• PLC-gamma1 verses PLC-gamma2
• Measurement of protein and enzymatic activation
• Downstream signaling by PLC-gamma in T cells
• PLC-gamma mediation of integrins during adhesion and spreading
• PLC-gamma biologic roles

Links

Series:

• Signal Transduction via Protein Tyrosine Kinase Receptors

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Prof. Graham Carpenter has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
• Ms. Cornelia Crooke has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.