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1. Introduction
2. Receptor tyrosine kinases
3. Within seconds to minutes of binding ligand
4. Within minutes to hours of binding ligand
5. Within 6-8 hours of binding ligand
6. Signaling events become more complex
7. Summary
8. The PDGFR and some of its associated proteins
9. Approach
10. The 'add-back' series of PDGFR mutants
11. PDGFR mutants induced DNA synthesis response
12. Conclusions
13. Dogma regarding growth factors and cell cycle
14. How close was the dogma to reality?
15. Continuous stimulation is necessary for S phase
16. Weakness of receptor mutant strategy
17. Experiments with PI3K inhibitors
18. The results with PI3K inhibitors
19. The meaning of the results
20. PI3K activity was necessary in mid G1
21. Are signaling enzymes active at later times?
22. PDGF triggered 2 waves of PI3K products
23. Late wave of PI3K contributes to mitogenic response
24. PI3K inhibitors blocked DNA synthesis
25. Why did PI3K inhibitors fail to block DNA synthesis
26. Wortmannin inhibited PI3K temporarily
27. Another approach
28. Conclusions
29. Waves of signaling raised an interesting question
30. Discontinuous stimulation strategy
31. Conclusion
32. Competence/progression
33. Rediscovery of competence/progression
34. G1 phase consists of two segments
35. How does priming/completion relate to cell cycle?
36. G1 cell cycle (c.c) program
37. Priming was insufficient to engage the cell cycle
38. The cell cycle was active during completion phase
39. Conclusions
40. How do cells know what time it is?
41. C.c response without the continuous exposure
42. Conclusions
43. Temporal regulation
44. Summary

Topics Covered

• How is signaling linked to cell cycle events?
• Recent findings that growth factors trigger multiple waves of signaling
• Engaging the cell cycle program by late wave signalling
• The hypothesis that growth factors trigger at least two distinct phases of signaling, and that each of them makes a unique contribution to cell cycle progression
• Appreciation of how growth factor-induced signaling drives the cell out of quiescence and commits it to one round of the cell cycle

Links

Series:

• Signal Transduction via Protein Tyrosine Kinase Receptors

Categories:

• Cancer
• Cell Biology
• Reproduction & Development

Therapeutic Areas:

• Oncology

Talk Citation

Kazlauskas, A. (2007, October 1). How the PDGF receptor induces cell proliferation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 18, 2024, from https://hstalks.com/bs/450/.
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Publication History

• Published on October 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Dr. Andrius Kazlauskas has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.