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1. Introduction
2. Early view of Ca2+ signaling
3. Phylogenetic tree of eukaryotic Ca2+-ATPases
4. Current view of Ca2+ signaling
5. Why have a Ca2+ pump in the Golgi?
6. Studies in yeast
7. Deletion of pmr1 in yeast: phenotypes
8. Ca2+ signaling in yeast
9. Reverse genetics: from gene to protein to function
10. Engineering the host
11. Overexpression
12. Purification of (His)9-Pmr1
13. Ion-dependent ATPase activity
14. Calcium transport in Golgi fractions
15. Phenotype screening
16. Phenotype screen for Ca2+/Mn2+ transport activity
17. Growth screens of mutants
18. Ion selectivity mutants
19. Q783A transports Ca2+ but not Mn2+
20. Intragenic suppression
21. Compensation of Q783A phenotype
22. Molecular modeling: from structure to function
23. SERCA/ SPCA Ca2+/ Mn2+ binding sites
24. Alignment between PMR1 and SERCA1a
25. Ca2+ binding site
26. Conformation-sensitive packing of helices
27. Cross-sectional view of core helices
28. Space-filled view of core helices
29. Golgi pumps in mammals
30. SPCA1 characteristics
31. Cell-cell adhesion lost in Hailey-Hailey disease
32. hSPCA1 complements pmr1 null phenotypes
33. Molecular basis of disease: from yeast to human
34. hSPCA1 localizes to the Golgi
35. Ca2+ transport activity
36. hSPCA1 transports Ca2+ with a high affinity
37. All Ca2+ ATPases confer BAPTA tolerance
38. Only PMR1 and hSPCA1 confer Mn2+ tolerance
39. Ca2+-transport activity of SPCA pumps
40. HHD mutants in yeast lacking endogenous pumps
41. HHD missense mutations
42. Localization of mutations in the homology model
43. SPCA1 distribution in polarized cells
44. SPCA2 has a more restricted tissue distribution
45. Primary hippocampal neurons
46. Organellar Ca2+ pumps
47. Acknowledgements

Topics Covered

• Early and current views on Calcium signaling
• Phylogenetic tree of Calcium-ATPases
• Identification of a new family of Golgi/secretory pathway Calcium-ATPases (SPCA)
• Why have a Calcium pump in the Golgi?
• Yeast as a model to understand Golgi Calcium pumps
• Biochemical characterization of Calcium and Mn2+ transport
• Phenotype screening in yeast to identify residues important for ion transport and selectivity in Pmr1
• Structural and homology models of Calcium-ATPases
• Golgi pumps in mammals
• From structure to physiology
• Haploinsufficiency of ATP2C1 leads to Hailey Hailey disease
• Insights from yeast
• Distribution and localization of SPCA1 and SPCA2

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Rao, R. (2007, October 1). Organellar calcium pumps of the golgi and ER: from yeast to human [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/IKZS3897.
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Publication History

• Published on October 1, 2007
• Archived on February 25, 2021

Financial Disclosures

• Prof. Rajini Rao has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.