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0:00
My name is Reinhard Grisshammer.
I am at the National Cancer Institute,
at the National Institutes of Health in Bethesda, Maryland.
The title of my talk is
structural and mechanistic insights into the neurotensin receptor.
0:20
The outline of my talk is as follows;
I will give a brief introduction into GPCRs,
G protein-coupled receptors and the neurotensin NTSR1.
Then, I will talk about conformational thermostabilization,
which is a technique to make proteins more amenable for crystallization.
Then, I will present four neurotensin receptors structures.
The first one is called NTSR1-GW5,
which has an active-like conformation and
provides insight into the neurotensin peptide binding mode.
Two more structures called NTSR1-ELF and LF
provide insight into the structural prerequisites of G protein activation.
The last structure is of a construct called EL which shows constitutive activity,
where we can learn about the structural features of a constitutively active receptor.
In the end, I will summarize constitutive and
agonist-induced signaling of the neurotensin receptor NTSR1.