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1. Introduction
2. Outline of the lecture
3. Characteristics of the family Bunyaviridae
4. Bunyaviridae genera
5. Principal Bunyavirus vectors
6. Cryoelectron microscopy of a Bunyavirus
7. Bunyavirus virion structure
8. Disease syndromes associated with Bunyaviridae
9. Oropouche Orthobunyavirus
10. La Crosse Orthobunyavirus
11. Hantaviruses
12. Hantaviruses in the old world
13. Hantavirus pulmonary syndrome
14. New world Hantaviruses
15. Rift valley fever Phlebovirus
16. Crimean-Congo haemorrhagic fever virus
17. Life-cycle of mosquito transmitted Orthobunyavirus
18. Virus is present in all stages of mosquito life cycle
19. Scanning EM of mosquito proboscis
20. Bunyavirus cycles
21. Transmission and maintenance cycles of the virus
22. Vector competence
23. Bunyavirus evolution
24. Bunyavirus reassortment
25. Occurrence of bunyaviruses reassortment
26. Bunyavirus reassortants
27. Garissa/Ngari virus
28. Orthobunyavirus replication
29. Replication of Bunyamwera virus in mosquito cells
30. Time course of viral protein synthesis
31. Transcription and replication of viral genome
32. Bunyavirus minireplicon system
33. Viral vector expression in the minireplicon system
34. Bunyavirus rescue: 3 plasmids
35. Creation of BUN MLM virus
36. BUN MLM virus forms small plaques
37. BUN MLM virus makes less L protein
38. BUN MLM virus packages less L segment RNA
39. BUN MLM virus has high particle:pfu ratio
40. BUN MLM virus is attenuated in mice
41. Orthobunyavirus S segment coding strategy
42. Deletion of BUNV NSs protein
43. Production of Bunyamwera virus lacking NSs
44. NSs is an interferon antagonist (1)
45. Interferon induction by BUNdelNSs
46. NSs is an interferon antagonist (2)
47. Summary of BUNdelNSs characteristics
48. Host cell shutoff by BUNdelNSs - mammalian cells
49. Monitoring shut-off in BUNV infected cells
50. Independent ability of NSs to cause shut-off
51. Dose dependant manner of shut-off by NSs
52. NSs and host cell shut-off
53. NSs has an effect on RNA polymerase II levels
54. How does NSs mediate its effect?
55. NSs-Med8 interaction
56. Med8 is a component of mediator
57. Mapping the interaction on NSs
58. Importance of C-terminus of NSs
59. Effect of truncated NSs on host protein shut-off
60. Effect of truncated NSs on RNA Pol II levels
61. Effect of truncated NSs on IFN-beta promoter
62. Induction of IFNbeta mRNA by NSs-mutant viruses
63. Conclusions about NSs
64. Function of NSs in mosquito cells
65. What about other Bunyaviruses?
66. Rift Valley fever virus NSs protein

Topics Covered

• Classification of bunyaviruses
• Diseases caused by bunyaviruses
• Natural history of bunyaviruses
• Bunyavirus evolution
• Bunyavirus replication
• Bunyavirus reverse genetics
• Bunyaviruses and the host innate immune response

Links

Series:

• Viruses and Viral Diseases

Categories:

• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on October 28, 2018

Financial Disclosures

• Prof. Richard Elliott has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.