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Printable Handouts
Navigable Slide Index
- Introduction
- Disulfide bond formation is an oxidation reaction
- Importance of disulfide bonds
- Disulfide bonds are formed in E. coli periplasm
- Chris Anfinsen and thermodynamic hypothesis
- De novo disulfide formation in E. coli periplasm
- The secretory pathway
- Disulfide bonds formation by ER thiol oxidases
- A model for QSOX-PDI cooperation
- Eukaryotic/prokaryotic disulfide bond formation
- A new pathway for disulfide bond formation
- DsbA and thioredoxin are structurally related
- DsbA and DsbB deletion and replacement
- Producing more oxidizing thioredoxin mutants
- Motility requires disulfide bonds
- 2 mutants can partially rescue dsb- phenotype
- Mutant protein is brown
- Absorbance spectrum CACC
- Iron-sulfur clusters
- Mutant thioredoxin's properties
- Mutant has two exposed cysteines
- Replacing normal pathway with a new pathway
- DsbB's quinone reductase activity
- Current model for the mechanism of DsbB
- Kinetics of the DsbB reaction
- Single wavelength kinetic measurement
- k1 depends on DsbA concentration
- k2 is fairly DsbA independent
- Kinetic measurements at 275 nm
- Kinetic scheme for DsbB reaction cycle
- Disulfide bond formation in prokaryotes
- Alchemy
- E. coli - the modern alchemist
- De novo disulfide formation and isomerization
- What distinguishes DsbC from DsbG?
- Annie Hiniker
- dsbC null mutants are copper sensitive
- WT DsbG doesn't rescue dsbC- Cu sensitivity
- Evolution of DsbG so it functions as DsbC
- DsbG K113E reverses electrostatic charge
- DsbG V216M mutant alters alpha c loop position
- DsbG V216M mutant reverses charge
- Acidic patch mutations in lysine 113
- Summary
Topics Covered
- Disulfide bond formation in prokaryotes
- In eukaryotes
- An engineered pathway
- DsbB, the source of disulfides
- Disulfide isomerases
Talk Citation
Bardwell, J. (2007, October 1). Disulfide bond formation in vivo [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 4, 2024, from https://doi.org/10.69645/CYHN8806.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. James Bardwell has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.