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1. Introduction
2. Disulfide bond formation is an oxidation reaction
3. Importance of disulfide bonds
4. Disulfide bonds are formed in E. coli periplasm
5. Chris Anfinsen and thermodynamic hypothesis
6. De novo disulfide formation in E. coli periplasm
7. The secretory pathway
8. Disulfide bonds formation by ER thiol oxidases
9. A model for QSOX-PDI cooperation
10. Eukaryotic/prokaryotic disulfide bond formation
11. A new pathway for disulfide bond formation
12. DsbA and thioredoxin are structurally related
13. DsbA and DsbB deletion and replacement
14. Producing more oxidizing thioredoxin mutants
15. Motility requires disulfide bonds
16. 2 mutants can partially rescue dsb- phenotype
17. Mutant protein is brown
18. Absorbance spectrum CACC
19. Iron-sulfur clusters
20. Mutant thioredoxin's properties
21. Mutant has two exposed cysteines
22. Replacing normal pathway with a new pathway
23. DsbB's quinone reductase activity
24. Current model for the mechanism of DsbB
25. Kinetics of the DsbB reaction
26. Single wavelength kinetic measurement
27. k1 depends on DsbA concentration
28. k2 is fairly DsbA independent
29. Kinetic measurements at 275 nm
30. Kinetic scheme for DsbB reaction cycle
31. Disulfide bond formation in prokaryotes
32. Alchemy
33. E. coli - the modern alchemist
34. De novo disulfide formation and isomerization
35. What distinguishes DsbC from DsbG?
36. Annie Hiniker
37. dsbC null mutants are copper sensitive
38. WT DsbG doesn't rescue dsbC- Cu sensitivity
39. Evolution of DsbG so it functions as DsbC
40. DsbG K113E reverses electrostatic charge
41. DsbG V216M mutant alters alpha c loop position
42. DsbG V216M mutant reverses charge
43. Acidic patch mutations in lysine 113
44. Summary

Topics Covered

• Disulfide bond formation in prokaryotes
• In eukaryotes
• An engineered pathway
• DsbB, the source of disulfides
• Disulfide isomerases

Links

Series:

• Molecular Chaperones

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. James Bardwell has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.