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1. Introduction
2. Bacillus subtilis
3. Proteolysis
4. ATP dependent proteases
5. Properties of Hsp100/Clp proteins
6. Model for ATP dependent proteases function
7. Properties of ClpC from B. subtilis
8. How is ClpCP involved in different processes?
9. Involvement of ClpCP in regulation
10. Competence development in B. subtilis
11. DNA uptake during transformation
12. ComK: master regulator/key transcription factor
13. ComK controlled by regulatory proteolysis
14. Involvement of ClpC in protein quality control (1)
15. Chaperone network and quality control in E. coli
16. Involvement of ClpC in protein quality control (2)
17. Does ClpC exhibit chaperone properties?
18. ClpC and MecA form bipartite chaperone system
19. MecA enables ClpCP to degrade proteins
20. What is the role of MecA?
21. MecA influence on ClpC
22. MecA enables oligomerization of the active ClpC
23. MecA targets substrate and activates ClpC
24. What about ClpP?
25. Formation of ClpCP complex is MecA dependent
26. Proteolytic active ClpCP/MecA complex
27. Adaptor proteins for ClpC
28. Are there more adaptor proteins for ClpC?
29. McsB acting as a regulated adaptor for ClpC
30. McsB: a tyrosine kinase
31. McsB
32. McsB is a kinase
33. McsB can act as a stress sensor
34. Competition of McsB and MecA for ClpC
35. Titration model of McsB kinase activity activation
36. McsB: adaptor protein targeting CtsR
37. McsB targets CtsR for degradation by ClpCP (1)
38. McsB targets CtsR for degradation by ClpCP (2)
39. Phosphorylated McsB can outcompete MecA
40. Expanded model: Hsp regulation of CtsR activity
41. B. subtilis ClpC adaptor protein network
42. Acknowledgements

Topics Covered

• Adaptor protein activation of ClpCP mediated proteolysis in B. subtilis Hsp100/Clp proteins and proteolysis
• ClpCP of B. subtilis in competence development and protein quality control
• Adaptor protein (MecA) mediated activation of ClpCP
• McsB a new regulated adaptor protein
• Adaptor protein network for ClpCP of B. subtilis

Links

Series:

• Molecular Chaperones

Categories:

• Biochemistry
• Cell Biology
• Microbiology

Talk Citation

Turgay, K. (2007, October 1). Adaptor mediated activation of the Hsp100/ Clp protein ClpC controls general and regulatory proteolysis in Bacillus subtilis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 25, 2024, from https://hstalks.com/bs/334/.
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Publication History

• Published on October 1, 2007
• Archived on February 25, 2021

Financial Disclosures

• Prof. Dr. Kursad Turgay has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.