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1. Introduction
2. The concept of apoptosis
3. Developmental cues induce cell death (1)
4. Interleukin-1β converting enzyme (ICE) (1)
5. Interleukin-1β converting enzyme (ICE) (2)
6. Developmental cues induce cell death (2)
7. Cystein aspartate-cleaving proteases-caspases
8. Developmental cues induce cell death (3)
9. What's special about proteases?
10. Proteases activity is irreversible
11. Protease regulation (1)
12. Example: blood clotting cascade
13. Blood clotting pathways
14. Protease regulation (2)
15. How do caspases disassemble a cell? (1)
16. Cell disassembly is like a military operation
17. Human caspases -3 and -7
18. DNA is cleaved during apoptosis
19. How is DNA cleaved?
20. Human caspase-6
21. Caspase-6 activity
22. Effector caspases -3, -6, and -7
23. Cell death is regulated by caspase-3
24. Activation of caspases by proteolytic processing
25. Detecting ICE activity
26. ICE can be isolated by affinity labeling
27. Detecting active caspases by precursor cleavage
28. Activation of caspases -3 and -7
29. Allosteric regulation of caspases
30. Caspases can activate other caspases
31. Human caspase-9
32. Activation of caspase-9
33. Apaf-1 is a homologue of CED-4
34. Caspase-9 holoenzyme
35. Induced proximity model
36. Caspases have domains that bind other proteins
37. Blood clotting model
38. Induced activity model
39. Activation of caspase-9 via cytochrome-c
40. Cytochrome-c and caspase cascade
41. Caspase cascade and coagulation cascade
42. Human caspase-8
43. Caspase-8 recruitment and activation
44. Two cell-death pathways – caspases-8 and -9
45. Caspase inhibitors
46. Caspase inhibitor pathways
47. Intrinsic and extrinsic pathways
48. Cell death activation
49. Why do cells that die by apoptosis look alike?
50. Thank you

Topics Covered

• What makes apoptotic cells alike?
• What are caspases?
• From the worm to humans
• What is special about proteases?
• What do blood clotting and apoptosis have in common?
• How do caspases disassemble a cell?
• How do you find out if caspases are active?
• How are caspases activated?
• What is left to learn?

Links

Series:

• Apoptosis

Categories:

• Cell Biology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Yuri Lazebnik has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.