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Printable Handouts
Navigable Slide Index
- Introduction
- TRP proteins form cation channels
- Table of TRP channels
- TRPM4 & TRPV6
- Expression of TRPM4 transcripts
- TRPM4 forms Ca2+-activated cation channels
- Increased Ca2+ influx in TRPM4 KO cells
- Explaining the increased Ca2+ influx (1)
- Explaining the increased Ca2+ influx (2)
- TRPM4 expression in the cardiovascular system
- Elevated blood pressure in TRPM4 KO mice (1)
- Circadian rhythm of TRPM4 KO mice
- RAAS in TRPM4 KO mice
- Fluid homeostasis in TRPM4 KO mice
- Elevated blood pressure in TRPM4 KO mice (2)
- Elevated blood pressure in TRPM4 KO mice (3)
- Contractility measurements of papillary muscle
- Regulation of blood pressure
- Contractility measurements in resistance vessels
- Contractility of resistance vessels & epinephrine
- Myogenic response in TRPM4 KO mice
- TRPM4 KO mice similarity to WT mice
- Cause for elevated BP in TRPM4 KO mice (1)
- Cause for elevated BP in TRPM4 KO mice (2)
- Metabolism of catecholamines
- Cause for elevated BP in TRPM4 KO mice (3)
- Expression of TRPM4
- TRPM4 and chromaffin cells
- TRPM4 transcripts in cells of the adrenal medulla
- Histology of the adrenal gland
- Detection of catecholamines using amperometry
- Amperomety in TRPM4 KO chromaffin cells
- Summary of the effects of TRMP4 KO
- TRPs and fertility
- TRPV6 proteins form Ca2+ selective ion channels
- Potential roles of TRPV6 in Ca2+ homeostasis (1)
- Potential roles of TRPV6 in Ca2+ homeostasis (2)
- Strategies to inactivate TRPV6 proteins
- Abolished Ca2+ conductivity of TRPV6(D541A)
- Generation of TRPV6(D541A) mice (1)
- Generation of TRPV6(D541A) mice (2)
- TRPV6(D541A) mice show normal weight gain
- Potential roles of TRPV6 in Ca2+ homeostasis (3)
- Intestinal Ca2+ absorption & homeostasis (1)
- Intestinal Ca2+ absorption & homeostasis (2)
- TRPV6(D541A) & bone architecture
- TRPV6(D541A) & bone mineralisation
- TRPV6(D541A) males are hypofertile (1)
- TRPV6(D541A) males are hypofertile (2)
- Spermatogenesis in mammals
- TRPV6(D541A) & sperm development
- Motility of TRPV6-deficient spermatozoa
- Motility of TRPV6(D541A) spermatozoa (1)
- Motility of TRPV6(D541A) spermatozoa (2)
- Reduced fertility of TRPV6(D541A) spermatozoa
- TRPV6 expression in the reproduction tract
- TRPV6 not expressed in testicular germinal layer
- TRPV6 is expressed in epididymal epithelia (1)
- TRPV6 is expressed in epididymal epithelia (2)
- Expression of TRPV6 in epididymal epithelium
- Caput epididymis
- Cauda epididymis
- Reduced viability of sperms in cauda epididymis
- Ca2+ concentration in the epididymal tubulus
- TRPV6 is essential in the epididymal tubulus
- Impaired Ca2+ absorption in epididymis
- Time course of Ca2+ concentration in sperms
- Summary: inactivation of TRPV6 in mice (1)
- Summary: inactivation of TRPV6 in mice (2)
- Consequences of TRPV6 pore inactivation
- Motility of TRPV6 KO spermatozoa
- Infertility in Trpv6 KO and in Trpv6(D541A) mice (1)
- Mating analysis
- Infertility in Trpv6 KO and in Trpv6(D541A) mice (2)
- D541A mutation leads to TRPV6 inactivation
- Acknowledgments
- Collaborations
- Funding
Topics Covered
- TRP channels: regulation of blood pressure and fertility
- Transient receptor potential channels
- regulation of intracellular Ca2+ homeostasis by cation channels with diverse permeability profiles
- TRPM4 and catecholamine secretion from chromaffin cells
- TRPV6-mediated Ca2+ uptake in epididymal epithelium
- Ca2+ homeostasis in epididymal fluid and male fertility
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Talk Citation
Freichel, M. (2014, February 4). TRP channels with diverse permeability profiles: regulation of blood pressure and fertility [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 21, 2024, from https://doi.org/10.69645/ZOOY4557.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Marc Freichel has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
TRP channels with diverse permeability profiles: regulation of blood pressure and fertility
Published on February 4, 2014
35 min
A selection of talks on Gynaecology & Obstetrics
Transcript
Please wait while the transcript is being prepared...
0:00
I am professor Marc Friechel.
I'm working at the
Pharmacological Institute,
and I'm heading the Department
of General Pharmacology.
Welcome to my talk with the
title, TRP Channels with Diverse
Permeability Profiles: Regulation
of Blood Pressure and Fertility.
0:20
The TRP class of ion channels
consists of 6 sub-families, which
cover altogether 28 members,
at least in mice.
Each TRP protein consists of six
putative transmembrane domains,
and it is generally believed
that four of such TRP proteins
together form hetero or
homomultimeric channel complexes.
0:44
Most TRP channels are non
selective with a permeability
for calcium of a sodium below 10.
Therefore TRP channels are not
only important for calcium entry
across the plasma
membrane, but play also
an important role in electrogenesis,
regulating the driving force
for calcium entry via other
calcium permeable channels.
This table gives you an overview
about the permeability of channels
formed by individual TRP
proteins of the TRPC, TRPV,
and TRPM sub-family.
It should be emphasized that
most of this information
is based on studies
of heterologously
expressed channel proteins.
And that characteristics
of those channels
may differ significantly from
native channel complexes that exist
in primary cells, since the
ectopically expressed channel
proteins do not necessarily
act in accordance
with the native cellular
context as in primary sets.
Within the TRP family
of cation channels
the highlighted channel
proteins TRPV5 and TRPV6,
as well as TRPM4 and TRPM5, have
distinct characteristic features.
TRPV6 proteins for instance, are
able to form channels that conduct
calcium ions with the
selectivity of calcium
over sodium of about more than 100.
Whereas TRPM4 protein similar
like TRPM5 from cation channels
that selectively conduct
monovalent cations.
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