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Printable Handouts
Navigable Slide Index
- Introduction
- Tracking gene expression
- Tracking post-translational modifications
- Calcium signaling in yeast (1)
- Calcium controls Crz1 localization
- Crz1 localization dynamics
- Extracting localization traces
- Initial observations: adaptation
- Tau delay
- Initial observations: autocorrelation
- Tau cluster
- Calcium increases Crz1 localization
- Crz1 localization is frequency-modulated
- FM versus AM radio
- FM versus AM localization
- Crz1 encodes signals via FM
- Transcriptional consequences of Crz1 bursts
- Consequences of bursts
- Two target genes with different input functions
- AM produces disproportional expression
- FM produces proportional expression
- FM, but not AM, causes proportional expression
- Synthetic promoters
- Natural targets of Crz1 (1)
- Natural target selection criteria
- 10 natural targets (1)
- All natural genes
- ‘Failed’ natural genes
- 10 natural targets (2)
- Overexpression perturbation
- Overexpression data
- Natural targets of Crz1 (2)
- Calcium-induced non-Crz1 targets
- Calcium signaling in yeast (2)
- Calcium pulses in mammalian cells
- Intracellular calcium measurements (1)
- Intracellular calcium measurements (2)
- Sample FRET traces
- Crz1 simplified diagram
- Calcineurin binding affinity
- Hints from other systems
- NFAT signaling in development and disease
- Acknowledgements
- Crz1 monster
Topics Covered
- Calcium and Signaling
- Excessive calcium causes cellular stress
- Signaling pathways are turned on to combat this stress
- In budding yeast, these pathways involve nuclear translocation of transcriptional regulator Crz1
- Examination of this regulator shows that nuclear translocation occurs in "bursts"
- Bursts are quantized and frequency-modulated
- Frequency modulation enables proportional expression of downstream target genes
- Update interview: Localization pulsing is general
- Update interview: Localization pulsing helps regulators control ~1000s of genes at the same time
Talk Citation
Dalal, C. (2020, May 15). Dynamic signal encoding in the S. cerevisiae calcium response [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 7, 2024, from https://doi.org/10.69645/QMBD6813.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Chiraj Dalal has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Update Available
The speaker addresses developments since the publication of the original talk. We recommend listening to the associated update as well as the lecture.
- Full lecture Duration: 46:23 min
- Update Interview Duration: 10:30 min
A selection of talks on Biochemistry
Transcript
Please wait while the transcript is being prepared...
0:00
Interviewer: Hello, Dr. Dalal,
thank you very much for doing
this update interview with us today.
Where we are going to discuss the changes
in the field of dynamic signaling encoding
in the Saccharomyces
cerevisiae calcium response.
We are also going to cover
the implications of this new knowledge and
where you see the fields headed.
So to start, what do we now know
on this topic that we did not
know at the time of
recording your talk in 2013?
Dr. Dalal: I think the major thing that
we know now that we didn't know then is
that, what happens in calcium signaling
is not limited to just calcium signaling.
What I mean by that is that at the time,
the transcriptional regulator CRZ 1 or
0:42
"crazy one" which is responsible for
calcium signaling was shown to have
these pulses of nuclear localization.
And since then we've now, there's been in
the field identified at least ten other
transcription factors that also pulse.
And moreover, all of them have also
been characterized in some depth.
So now we know that this is not just
isolated to calcium signaling, but
that this sort of signal encoding
occurs all across the yeast genome.
And more importantly than that,
we also know that orthologs of
the CRZ 1 transcriptional regulator,
notably NF-AT in mammalian cells also
show this sort of pulsatile behavior.
And so this sort of dynamic single
cell analysis has really enabled us
to see how different ways that biological
systems can regulate themselves.
And it's just, it's not specific to
calcium that just happened to be
the place where we made
the identification first.
Interviewer: And so
what are the implications of this?
Dr. Dalal: I think the implications
are that we thought that this,
when we first discovered the crazy,
the CRZ 1 system,
we were really intrigued by the fact that
we see this frequency modulated behavior.