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1. Introduction
2. Mycobacteria phylogenetic tree
3. Slowly growing mycobacteria
4. Evolutionary success of M. tuberculosis: genetics
5. Genome of M. tuberculosis H37Rv
6. M. tuberculosis H37Rv - 4000 genes annotated
7. M. tuberculosis H37Rv - zoom-in
8. Broad classification of M. tuberculosis genes
9. Identifying the essentials genes of M. tuberculosis
10. 600 essential genes predicted using TraSH
11. High density transposon mutation screens & NGS
12. Example for an essential gene - pknB
13. M. tub. serine threonine protein kinases (STPK)
14. Genomic region pknB - a conserved operon
15. PknB is essential for M. tuberculosis
16. Identifying virulence factors
17. Mycobacterial survival - genetic requirements
18. Genes encoding ESX-1 type 7 secretion system
19. Other ways to predict genes involved in virulence
20. Mycobacterium bovis BCG
21. Mycobacterium microti
22. Region of difference 1 (RD1)
23. RD1 positive vs. RD1 negative proteomes
24. BCG-RD1 different colony morphology than control
25. The RD1 region codes for ESX-1 (1)
26. The RD1 region codes for ESX-1 (2)
27. Novel gene nomenclature for T7SS - ESX-1
28. Re-integration of RD1 region into BCG
29. ESX-1 mediated processes differ in BCG & M. tub.
30. M. tuberculosis has 5 ESX/type VII systems
31. Comparative genomics within the tubercle bacilli
32. Host range of tubercle bacilli
33. Comparative genomics identifies RDs
34. Sequence conservation of the RD9 junction region
35. Comparative genomics identifies TbD1
36. TbD1 absent from many M. tuberculosis strains
37. Deletion events and the M. tuberculosis complex
38. Evolutionary pathway of the tubercle bacilli
39. M. tub. variants: presence around the world
40. The oldest case of human tuberculosis (in Britain)
41. Broad diversity of worldwide M. tuberculosis (1)
42. Broad diversity of worldwide M. tuberculosis (2)
43. Conclusions and perspectives
44. Acknowledgements

Topics Covered

• Mycobacterium tuberculosis
• genome information
• essential genes- virulence genes
• type VII secretion system involved in virulence and host pathogen interaction- genomics and evolution of tuberculosis-causing mycobacteria

Links

Series:

• Tuberculosis

Categories:

• Genetics & Epigenetics
• Microbiology

Therapeutic Areas:

• Infectious Diseases
• Respiratory Diseases

Talk Citation

Brosch, R. (2013, February 28). The genetic structure of Mycobacterium tuberculosis - insights into genome organisation and evolution of the causative agent of tuberculosis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/RSOZ2298.
Export Citation (RIS)

Publication History

• Published on February 28, 2013

Financial Disclosures

• Dr. Roland Brosch has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.