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Biomedical Basics

Flow cytometry

  • Created by Henry Stewart Talks
Published on July 30, 2026   4 min

A selection of talks on Haematology

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In this talk, we turn our attention to flow cytometry, framing our discussion around the principles of flow cytometry, including its fluidics, optics and electronic systems that enable rapid multi parameter analysis of cells. We will discuss essential sample preparation techniques, selection of antibodies and florochromes, and key data acquisition steps. The presentation will also address data analysis methods, common applications, and current limitations of the technology. Lastly, we will highlight the importance of proper experimental design and compensation in achieving robust reproducible results. Flow cytometry is a powerful technique used to assess the physical and chemical characteristics of cells or particles as they flow in suspension through a beam of light. It comprises three components, fluidics, which transports and focuses cells, so they pass single file, Optics, which uses lasers and lenses to illuminate cells and collect signals and electronics, which processes signals into digital data. This system enables rapid multi parameter analysis of thousands of cells per second, revealing cellular diversity. Preparing samples properly is critical in flow cytometry. Most applications require a single cell suspension from tissues, blood or cultured cells, often using mechanical or enzymatic methods. Debris and aggregates must be avoided to maintain data quality. Cells are then incubated with florochrome conjugated antibodies that bind to specific markers.

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