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1. Introduction
2. AIST
3. Biological functions of glycans
4. Glycoproteome is the future
5. Glycoproteomics
6. Glycans are clothes for cells (proteins)
7. Variety of monosaccharide
8. N-glycans and O-glycans
9. Human ABO blood antigens
10. Three essentials of glycoscience
11. Glycogene project (GG project)
12. The strategy of cloning for glycogene
13. GG project (2001-2004)
14. Glycogene family cloned and analyzed to date
15. Structural glycomics project (SG project)
16. Complexity of glycan structures
17. Glycan synthesizer
18. Examples for glycan synthesizer performance
19. Methods for glycan structure analysis
20. Multistage tandem mass spectrometry
21. Multi-stage tandem mass spectrometry example
22. Glycogene expression profiling
23. Evanescent-field fluorescence-assisted scanner
24. IGOT LC/MS method
25. Our technology for analyzing glycans
26. Medical glycomics project (MG project)
27. Functions of glycans
28. Glyco-biomarkers for liver fibrosis and cancer
29. The development of glycoprotein biomarker
30. Glycans as serum markers for cancer
31. Glycans methods for cancer seromarkers
32. AGP as a liver fibrosis marker
33. How to succeed in biomarker development?
34. Pathology of liver fibrosis
35. The prevalence of HCV infection
36. Disease severity in HCV infected patients
37. Issue in gold standard procedure "liver biopsy"
38. Issues in current non-invasive system
39. Our motivation
40. Lectin microarray system
41. Fibrosis-related fucosylation in AGP
42. Serum AGP method
43. Differential glycan profiling
44. Lectin as diagnostic tool for liver cirrhosis
45. Transfer to automatic assay system
46. Multi-cohort study in Japan (2009-2010)
47. Comparison of diagnostic performance
48. Biomarkers of cancer
49. Useful biomarkers for diagnosis (1)
50. Useful biomarkers for diagnosis (2)
51. Development of bile duct cancer marker
52. Development of glycan profiling method
53. Marker targeting for tissue sections
54. Diagnostic by MY.1E12 antibody and WFA lectin
55. Novel glyco-biomarker for cholangiocarcinoma
56. WFA-MY.1E12 sandwich ELISA (bile)
57. Specific glycoprotein marker
58. Development of glyco-biomarker
59. Conclusions
60. Construction of glycan-related database

Topics Covered

• Overview of glycans
• Developing the technologies used in glycoscience
• Examples of successful glycobiomarker development

Links

Series:

• Biomarkers
• Glycobiology

Categories:

• Biochemistry
• Cell Biology
• Clinical Practice

Talk Citation

Narimatsu, H. (2012, October 11). Development of glyco-biomarkers using glycoproteomics technologies [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved March 25, 2023, from https://hstalks.com/bs/2404/.
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Publication History

• Published on October 11, 2012

Financial Disclosures

• Dr. Hisashi Narimatsu, Grant/Research Support (Principal Investigator): Research grants since 2000 to 2011 every year funded by the government (New Energy and Industrial Technology Development Organization; NEDO) ; Constructions of Libraries of Human Genes Participating in Glycosylation (April 2001-March 2004, Glycogene: NEDO GG project) ; Development of Methodologies and Databases for Structural Glycoproteomics (April 2003-February 2—6, Structural Glycomics: NEDO SG project) ; Developing Technology for Implementing Sugar Chain Functions (April 2006-February 2011, Medical Glycomics: NEDO MG project) ; Research grants since 2007 to present every year funded by the government (ministry of Education, Culture, Sports, Science and Technology (MEXT) Integrated database project).