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Printable Handouts
Navigable Slide Index
- Introduction
- Introducing the cell boundary theorem
- Lecture plan
- Theorem history
- Influencing intracellular free calcium concentration
- Processes affecting steady-state cytosolic Ca2+
- Skeletal muscle excitation-contraction coupling (1)
- Skeletal muscle excitation-contraction coupling (2)
- Malignant hyperthermia
- Cytosolic Ca levels in malignant hyperthermia
- RyR1 and RyR3 affect cytosolic Ca levels
- Calsequestrin-1
- Three interventions increase cytosolic Ca levels
- Factors affecting free cytosolic Ca levels
- The cell boundary theorem explained
- RyR1-mediated Ca leak and entry
- Resting cation entry in RyR-null vs. Wt cells
- Cytosolic Ca levels following Ca buffer addition (1)
- A "non-calcium" application of the theorem (1)
- Lecture summary
- Acknowledgements
- A "non-calcium" application of the theorem (2)
- Cytosolic Ca levels following Ca buffer addition (2)
Topics Covered
- Cellular homeostasis
- Calcium movements
- Movements of Ions and Solutes
- A general property that helps determine steady state concentrations in cytosol and organelles
- Application to skeletal and cardiac muscle in health and disease
- Malignant Hyperthermia
- Use of calcium and pH monitoring dyes
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Talk Citation
Ríos, E. (2020, March 23). Cell boundary theorem [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 7, 2024, from https://doi.org/10.69645/ATPO9908.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Eduardo Ríos has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Update Available
The speaker addresses developments since the publication of the original talk. We recommend listening to the associated update as well as the lecture.
- Full lecture Duration: 29:54 min
- Update interview Duration: 9:43 min
A selection of talks on Biochemistry
Transcript
Please wait while the transcript is being prepared...
0:00
My name is Eduardo Rios.
I work in the section of cellular
signaling at Rush University in Chicago.
As you can see from this picture
taken in the boundary waters of
Northwest Wisconsin, the title of this
lecture is the cell boundary theorem.
The topic is an idea, actually
a principle of the physics that rules
the movement of solutes between cells and
their environment.
As we shall see later, this idea was
known by many researchers in the field of
calcium signaling, and it was probably in
this field that it was first understood.
Given that calcium was one of the first
signaling molecules to be studied and
thought about.
But it is not solely applicable to calcium
as it applies to all transported solutes.
In the lecture we will make sure
that you keep this in mind.
1:01
I thought of introducing this idea
formally in the literature two or
three years ago,
as I saw how often it was ignored or
just contradicted in published work.
I did it in a short paper published in
the Journal of Physiological Sciences.
In purposely formulating it as a theorem,
I meant to emphasize that it was true,
demonstrable from first principles and
therefore could not be violated.
The concept appears trivial to some, but
to others it feels surprising and
not immediately easy to grasp.
Of approximately 40 colleagues,
faculty and students who attend
the cell Signaling Journal Club in our
section about 10 found the idea trivial.
All others had various degrees of
difficulty with it, interestingly,
so did the three referees that
reviewed our manuscript for
the Journal of Physiological Sciences.