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Navigable Slide Index

1. Introduction
2. Measurements of cytokines
3. Features relevant to assay development
4. Types of assays for cytokines
5. Ex vivo assays for cytokines
6. The principle of ELISA
7. Assay selection is a critical step
8. Single-cell assays for intracellular expression
9. Intracytoplasmic cytokine staining (ICS)
10. "Cytokine screen" with PBMC
11. Enumeration of cytokine-secreting single T cells
12. Immunohistochemistry for TGF-beta1
13. Multiplex analyses
14. Multiplex bead array technology
15. How the Bio-Plex protein array system works
16. Cytokine profiling by xMAP
17. ELC platform
18. Protein expression or cytokine transcripts?
19. QC/QA issues: general principles
20. Good laboratory practice: QC
21. IL-12p70 production by human dendritic cells
22. Comparing commercial/in-house IL-12 standards
23. Data analysis in cytokine assays
24. The SPICE program
25. IL-12p70 levels established for DC products
26. Pitfalls of cytokine assays
27. Cytokines in biotherapy trials
28. Acknowledgement

Topics Covered

• The importance of measuring cytokines
• The types of cytokine assays available
• Examples of most widely used cytokine assays
• Principles of ELISA and ELISPOT
• How to select the right cytokine assay
• Multiplex analyses and cytokine profiling
• Principles of QA/QC for cytokine assays
• Data analysis and pitfalls

Links

Series:

• Cytokines

Categories:

• Immunology
• Methods

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Whiteside, T. (2011, December 5). Assays for cytokines and cytokine assay standardization [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/KSZI7822.
Export Citation (RIS)

Publication History

• Published on December 5, 2011
• Archived on January 31, 2022

Financial Disclosures

• Prof. Theresa Whiteside has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.