Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Overview
3. Acute lung injury
4. ALI and ARDS: consensus definition
5. ALI/ARDS and inflammation
6. Inflammation in acute lung injury
7. ALI: an inflammatory lung disease
8. The paradox
9. Selected basics of neutrophil biology
10. Hematopoiesis
11. Mature neutrophils
12. The neutrophil: king of phagocytosis
13. Phagocytosis (1)
14. Phagocytosis (2)
15. Neutrophil granules
16. Phagocyte NADPH oxidase (NOX2)
17. Neutrophil adhesion and emigration
18. Back to the lung
19. PMN sequestration mechanisms in ALI
20. PMN sequestration in pulmonary capillaries
21. PMN transiting a model capillary in vitro
22. Leukocyte sequestration in ARDS
23. Epithelial (epi.) function in lung injury
24. Mechanisms and consequences of PMN migration
25. Inter-epithelial cell junctions
26. Inter-epithelial (i-epi.) junctional molecules
27. In vitro model of PMN transmigration
28. PMN transepithelial migration
29. PMN migration creates areas of microinjury (1)
30. PMN migration creates areas of microinjury (2)
31. I-epi. junction disruption and neutrophil infiltration
32. Elastase and trans-epithelial resistance decrease
33. E-cadherin cleavage by PMN elastase
34. Inter-epithelial junctional molecules cadherins
35. Epi. injury mechanisms induces by PMN migration
36. Epithelial apoptosis following PMN transmigration
37. Elastase induces epithelial cell apoptosis (1)
38. Elastase induces epithelial cell apoptosis (2)
39. Mechanisms of elastase-induced apoptosis
40. Pathways of apoptosis
41. Elastase increases mitochondrial permeability
42. Elastase induces cleavage of caspases 9 and 3
43. Protease activated receptors
44. PAR-1 siRNA blocks elastase-induced apoptosis
45. Epithelial repair after ALI
46. PMN transmigration: epithelial injury and repair
47. Alterations in epithelial gene expression
48. Ingenuity pathway analysis (1)
49. Ingenuity pathway analysis (2)
50. Wnt/beta-catenin pathway
51. PMN transmigration induces beta-catenin signaling
52. PMN migration induces beta-catenin translocation
53. PMN transmigration induces TOPFlash activity
54. Beta-catenin genes upregulated by PMN migration
55. PMN migration and beta-catenin activation in vivo
56. Beta-catenin dependent transcription and PMNs
57. PMN migration induces cyclin D1 expression
58. Functional role of beta-catenin in injury and repair
59. ShRNA knockdown of beta-catenin
60. Beta-catenin in epithelial injury and repair
61. Conclusions
62. The concepts discussed
63. Therapeutic implications
64. Acknowledgements

Topics Covered

• Clinical problem of acute lung injury and ARDS
• physiological role of neutrophils in host defense and innate immunity
• pathological role of neutrophils in inflammatory tissue injury in general and in acute lung injury
• mechanisms of damage to the lung epithelium by neutrophils
• paradoxical role of neutrophils in initiating repair processes

Links

Series:

• Molecular Basis of Bacterial Infection

Categories:

• Clinical Practice
• Immunology

Therapeutic Areas:

• Immunology & Inflammation
• Respiratory Diseases

Talk Citation

Publication History

• Published on July 27, 2011

Financial Disclosures

• Prof. Gregory Downey has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.