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Printable Handouts
Navigable Slide Index
- Introduction
- Signal transduction - a short introduction
- Signal transduction resembles a wiring diagram
- Protein phosphorylation and protein kinase
- Diseases caused by mutations in protein kinases
- WNK kinase mutations cause hypertension (1)
- WNK kinase mutations cause hypertension (2)
- Identification of WNK1-interacting proteins
- SPAK and OSR1 are close relatives
- WNK1 phosphorylates and activates SPAK & OSR1
- CCT domain in SPAK/OSR1 binds WNK1
- SPAK/OSR1 phosphorylation regulates NKCC1
- CCT in SPAK/OSR1 also binds NKCC1 substrate
- The CCT domain binds to an RFXV motif peptide
- Conclusions (1)
- Regulation of WNK1 by hyperosmotic conditions
- Hyperosmotic stress activates NKCC1
- Hyperosmotic stress - WNK1 & SPAK/OSR1
- Hyperosmotic stress activates WNK1
- How osmotic stress induces WNK1 activation
- Phosphorylation sites in WNK1
- Regulation of WNK1-SPAK/OSR1 interactions
- Hyperosmotic stress activates SPAK/OSR1
- Conclusions (2)
- Thiazide diuretics affect blood pressure-model
- Is NCC phosphorylated by WNK-SPAK pathway?
- Analysis of NCC phosphorylation in mpkDCT cells
- Thr60 phosphorylation mediates NCC activation
- Summary (1)
- Defining the physiological roles of SPAK in KI mice
- Phosphorylation of NCC in the kidney in KI mice
- Arterial blood pressure measurements
- Reduced blood pressure in SPAK knock-in mice
- Conclusions (3)
- WNK1 and SPAK/OSR1 phosphorylate NKCC2
- Roles of MO25 beyond regulating LKB1
- STRADalpha interacts with MO25 (1)
- STE20 kinase family tree
- Effect of MO25 on 27 STE-kinases
- SPAK/OSR1 phosphorylates NCC and NKCC2
- MO25 enhances NKCC2/NCC phosphorylation
- MO25 binds to STRAD via two sites (Sites 1/2)
- Mutations in MO25 reduce SPAK activation
- STRADalpha interacts with MO25 (2)
- OSR1 activation by MO25
- Summary (2)
Topics Covered
- Mutations in WNK kinases cause familial hypertension
- WNK1 interacts with and activates SPAK and OSR1
- Physiological functions of SPAK and OSR1
- Hyperosmotic conditions affect NKCC1 via WNK1 and SPAK/OSR1
- A suggested mechanism of action for Thiazide diuretics in Gordon's syndrome patients
- How the WNK pathway regulates blood pressure-a current model
- Defining physiological roles of SPAK in knock-in mice
- MO25 also regulates the activity of SPAK/OSR1
- Functions and schematic representation of LKB1 and SPAK/OSR1 protein complexes
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Talk Citation
Alessi, D. (2011, March 16). WNK1 pathway and its role in regulating hypertension [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 12, 2024, from https://doi.org/10.69645/AIBZ8759.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Dario Alessi has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.