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1. Introduction
2. Bioelectrical signals
3. What is bioelectricity?
4. Defining bioelectricity
5. Big questions about bioelectricity
6. Sources of bioelectrical signals
7. Physiological side effect or instructive signal?
8. Membrane voltage and cell plasticity
9. History of the field
10. Take home messages
11. Outline
12. Applying exogenous electric fields in vitro
13. Microelectrode impalement
14. Detection of extracellular ion flux
15. Investigating signals in pattern formation
16. Inverse pharmacological screen
17. Transmembrane voltage gradient characterization
18. Loss- and gain-of-function analyses
19. Synthesizing data into a predictive model
20. Control of cell behavior by electric fields
21. Mammalian stem cell differentiation
22. Cell orientation and outgrowth
23. Transepithelial potentials, electric fields, cancer
24. Mechanisms of electric field sensing by cells
25. Tissue-level electric fields and their function
26. Electric fields and tissue repair
27. Bioelectric signals in 3D morphogenesis
28. Bioelectric signals in morphogenetic events (1)
29. Bioelectric signals in morphogenetic events (2)
30. Transmembrane potential in tail regeneration (1)
31. Transmembrane potential in tail regeneration (2)
32. V-ATPase function in tail regeneration
33. V-ATPase activity is required for tail regeneration
34. V-ATPase needed for endogenous regeneration
35. Using voltage- and pH-sensitive dyes
36. Induction of the V-ATPase following injury
37. Electrogenic targets in mitosis upregulation
38. Axon patterning requires specific ion fluxes
39. Gene expression requires specific ion flows
40. A pathway for tail regeneration
41. Can ion flux induce regeneration?
42. Summary (1)
43. Voltage gradients control large-scale axial polarity
44. Transmembrane potential and cell behavior
45. The Goldman equation
46. Missexpressing specific ion transporter mRNAs
47. Melanocyte behavior & transmembrane potential
48. Taking advantage of endogenous ion transport
49. Controlling transmembrane potential
50. Controlling proliferation rates using K channels
51. Properties of cells and transmembrane potential
52. Effects of transmembrane potential changes
53. Modulation of transmembrane potential
54. Molecular mechanisms
55. Cell-autonomous mechanisms
56. Non-cell-autonomous mechanisms:
57. Bioelectrical signals and canonical pathways
58. Properties of bioelectrical signaling
59. Bioelectric gradients and gene expression
60. Bioelectrical signals are fundamental
61. Bioelectrical signals exert long-range effects
62. Information carried by bioelectric signals
63. Ion channel genes may be irrelevant
64. Bioelectrical mechanisms, DNA, mRNA & protein
65. Control of ion flux is often a "master regulator"
66. Future prospects
67. Cell types live in a biophysical state space
68. Applications in cancer, stem cells & regeneration
69. Light-gated ion transporters
70. Summary (2)
71. Acknowledgements

Topics Covered

• Defining bioelectricity
• Sources of bioelectrical signals
• Big questions about bioelectricity
• Membrane voltage and cell plasticity
• History of the field of bioelectricity
• Applying exogenous electric fields in vitro
• Microelectrode impalement
• Detection of extracellular ion flux
• Investigating signals in pattern formation
• Inverse pharmacological screen
• Transmembrane voltage gradient characterization
• Loss- and gain-of-function analyses
• Synthesizing data into a predictive model
• Control of cell behavior by electric fields
• Mammalian stem cell differentiation
• Cell orientation and outgrowth
• Transepithelial potentials, electric fields, cancer
• Mechanisms of electric field sensing by cells
• Bioelectric signals in 3D morphogenesis
• A pathway for tail regeneration
• Transmembrane potential in tail regeneration
• V-ATPase function in tail regeneration
• Using voltage- and pH-sensitive dyes
• Induction of the V-ATPase following injury
• Electrogenic targets in mitosis upregulation
• Axon patterning requires specific ion fluxes
• Gene expression requires specific ion flows
• Ion flux and induction of regeneration
• Voltage gradients control large-scale axial polarity
• Transmembrane potential and cell behavior
• The Goldman equation
• Missexpressing specific ion transporter mRNAs
• Melanocyte behavior & transmembrane potential
• Taking advantage of endogenous ion transport
• Controlling transmembrane potential
• Modulation of transmembrane potential
• Controlling proliferation rates using K channels
• Cell-autonomous mechanisms
• Non-cell-autonomous mechanisms
• Bioelectrical signals and canonical pathways
• Bioelectric gradients and gene expression
• Information carried by bioelectric signals
• Bioelectrical mechanisms, DNA, mRNA & protein
• Control of ion flux as a "master regulator"
• Applications in cancer, stem cells & regeneration
• Light-gated ion transporters

Links

Categories:

• Cancer
• Cell Biology
• Reproduction & Development

Therapeutic Areas:

• Oncology

Talk Citation

Levin, M. (2011, February 23). Endogenous bioelectrical signals in development, regeneration, and neoplasm [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved May 29, 2023, from https://hstalks.com/bs/1946/.
Export Citation (RIS)

Publication History

• Published on February 23, 2011

Financial Disclosures

• Prof. Michael Levin has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.