• The Discovery of Protein Phosphorylation
• 45 min
  1. Phosphorylase and the origin of reversible protein phosphorylation

  • Prof. Edmond Fischer
• 21 min
  2. Three decades of protein phosphorylation and cancer: the identification and characterization of the src gene product

  • Prof. Raymond Erikson
• Protein Kinase Cascades
• 32 min
  3. Role of the PI 3-kinase signaling pathway in cell regulation and human disease

  • Prof. Lew Cantley
• The Modulation of Protein Function by Phosphorylation
• 33 min
  4. Two is the key to 14-3-3: dimeric mechanical signaling devices

  • Prof. Carol MacKintosh
• Protein Phosphatases
• 38 min
  5. Structure and mechanisms of protein phosphatases

  • Prof. David Barford
• 45 min
  6. Protein tyrosine phosphatases

  • Prof. Jack Dixon
• 49 min
  7. The regulation of MAP kinase signalling by dual-specificity protein phosphatases

  • Prof. Steve M. Keyse
• 22 min
  8. Protein phosphatase inhibitors: a distillation of cell signaling research and water toxicology

  • Prof. Carol MacKintosh
• The Structures of Protein Kinases
• 45 min
  9. Protein kinase structure, function and regulation

  • Prof. Susan Taylor
• 42 min
  10. The structural basis for the modulation of protein function by protein phosphorylation

  • Prof. Dame Louise N. Johnson
• Biological Systems that are Regulated by Reversible Phosphorylation
• 50 min
  11. Protein phosphorylation and the control of protein synthesis

  • Prof. Christopher Proud
• 62 min
  12. The role of phosphorylation in mediating cellular responses to DNA damage: the ATM-mediated DNA damage response

  • Prof. Yosef Shiloh
• 40 min
  13. Roles of AMPK in energy homeostasis and nutrient sensing

  • Prof. Grahame Hardie
• 56 min
  14. Serine kinases and T lymphocyte biology

  • Prof. Doreen Cantrell
• 30 min
  15. The interplay between protein phosphorylation and ubiquitylation in the NF-κB pathway

  • Prof. Zhijian 'James' Chen
• 58 min
  16. SMAD phosphorylation and the TGF-beta pathway

  • Prof. Joan Massagué
• Protein Kinases and Human Disease
• 58 min
  17. Function and regulation of the PDK1 kinase

  • Prof. Dario Alessi
• 63 min
  18. LKB1 pathway and its role in cancer

  • Prof. Dario Alessi
• 51 min
  19. WNK1 pathway and its role in regulating hypertension

  • Prof. Dario Alessi
• 33 min
  20. The hyperphosphorylation of tau and Alzheimer's disease

  • Prof. Michel Goedert
• Protein Kinases as Targets for the Development of Anti-Cancer Drugs
• 68 min
  21. PI3K/AKT signaling in cancer

  • Prof. Neal Rosen
• 37 min
  22. RAS and RAF signaling in melanoma: biology and therapies

  • Prof. Richard Marais
• 38 min
  23. The mTOR kinase as a target for anti-cancer drugs

  • Prof. David Sabatini
• Archived Lectures *These may not cover the latest advances in the field
• 51 min
  24. Modular protein-protein interactions provide a general mechanism to organize dynamic cellular systems

  • Prof. Tony Pawson
• 38 min
  25. AMP-activated protein kinase: regulating cellular and whole body energy balance

  • Prof. Grahame Hardie

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Kinases and phosphatases
3. Ways to remove phosphate from proteins
4. Class I Cys-based PTPs
5. Phosphatase families
6. Human genome
7. Mutational analysis of PTP-1
8. Sequences at active site (1)
9. Phospho-enzyme intermediate
10. pH stability of phosphoprotein phosphate
11. Site-directed mutational analysis of VHR
12. PTPs share a catalytic active site motif
13. Sequences at active site (2)
14. Sequences of phosphatases
15. Yop 2b
16. The bacterial Yop 2b phosphatase activity
17. Bacterial growth in the spleen of mice
18. Yop 2b in eukaryotic macrophages
19. Yersinia inject phosphatase into eukaryotic cells (1)
20. Yersinia inject phosphatase into eukaryotic cells (2)
21. Interaction between Yersinia and a target cell
22. Phosphatases as tumor suppressor genes
23. Lab members that researched PTEN
24. PTEN and myotubularin structure
25. PTEN - 10q23.3
26. Frequency of PTEN mutations
27. Kinetic constants of dual-specificity phosphates
28. PTEN is a "lipid" phosphatase
29. PTEN might inhibit cell survival
30. Effect of PTEN overexpression on PIP3 production
31. Effect of PTEN expression on PI 3-kinase activation
32. Inositol phosphatase activity of PTEN
33. PTEN tumor suppressor activity
34. PTEN and PIP3 researchers
35. RNA interference first developed in C.elegans
36. RNA interference protocol for fly S2 cells
37. Mammalian, C.elegans and fly signaling cascade
38. PTEN inhibits Akt activity
39. Crystal structure of PTEN
40. The active site pocket of PTEN
41. Sequence alignment of PTEN catalytic site
42. Structure of PTEN
43. Summary

Topics Covered

• Kinases and phosphatases
• Ways to remove phosphate from proteins
• Class I Cys-based PTPs
• Phosphatase families
• Mutational analysis of PTP-1
• Sequences at active site
• Phospho-enzyme intermediate
• pH stability of phosphoprotein phosphate
• Site-directed mutational analysis of VHR
• How PTPs share a catalytic active site motif
• Sequences of phosphatases
• The bacterial Yop 2b phosphatase activity
• Bacterial growth in the spleen of mice
• Yop 2b in eukaryotic macrophages
• Interaction between Yersinia and a target cell
• Phosphatases as tumor suppressor genes
• PTEN and myotubularin structure
• PTEN-10q23.3
• Frequency of PTEN mutations
• Kinetic constants of dual-specificity phosphates
• How PTEN is a "lipid" phosphatase
• How PTEN might inhibit cell survival
• Effect of PTEN overexpression on PIP3 production
• Effect of PTEN expression on PI 3-kinase activation
• Inositol phosphatase activity of PTEN
• PTEN tumor suppressor activity
• Key PTEN and PIP3 researchers -RNA interference in C.elegans
• RNA interference protocol for fly S2 cells
• Mammalian, C.elegans and fly signaling cascade
• How PTEN inhibits Akt activity
• The active site pocket of PTEN
• Sequence alignment of PTEN catalytic site

Links

Series:

• Protein Phosphorylation

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on November 30, 2010

Financial Disclosures

• Prof. Jack Dixon has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.