• The Discovery of Protein Phosphorylation
• 45 min
  1. Phosphorylase and the origin of reversible protein phosphorylation

  • Prof. Edmond Fischer
• 21 min
  2. Three decades of protein phosphorylation and cancer: the identification and characterization of the src gene product

  • Prof. Raymond Erikson
• Protein Kinase Cascades
• 32 min
  3. Role of the PI 3-kinase signaling pathway in cell regulation and human disease

  • Prof. Lew Cantley
• The Modulation of Protein Function by Phosphorylation
• 33 min
  4. Two is the key to 14-3-3: dimeric mechanical signaling devices

  • Prof. Carol MacKintosh
• Protein Phosphatases
• 38 min
  5. Structure and mechanisms of protein phosphatases

  • Prof. David Barford
• 45 min
  6. Protein tyrosine phosphatases

  • Prof. Jack Dixon
• 49 min
  7. The regulation of MAP kinase signalling by dual-specificity protein phosphatases

  • Prof. Steve M. Keyse
• 22 min
  8. Protein phosphatase inhibitors: a distillation of cell signaling research and water toxicology

  • Prof. Carol MacKintosh
• The Structures of Protein Kinases
• 45 min
  9. Protein kinase structure, function and regulation

  • Prof. Susan Taylor
• 42 min
  10. The structural basis for the modulation of protein function by protein phosphorylation

  • Prof. Dame Louise N. Johnson
• Biological Systems that are Regulated by Reversible Phosphorylation
• 50 min
  11. Protein phosphorylation and the control of protein synthesis

  • Prof. Christopher Proud
• 62 min
  12. The role of phosphorylation in mediating cellular responses to DNA damage: the ATM-mediated DNA damage response

  • Prof. Yosef Shiloh
• 40 min
  13. Roles of AMPK in energy homeostasis and nutrient sensing

  • Prof. Grahame Hardie
• 56 min
  14. Serine kinases and T lymphocyte biology

  • Prof. Doreen Cantrell
• 30 min
  15. The interplay between protein phosphorylation and ubiquitylation in the NF-κB pathway

  • Prof. Zhijian 'James' Chen
• 58 min
  16. SMAD phosphorylation and the TGF-beta pathway

  • Prof. Joan Massagué
• Protein Kinases and Human Disease
• 58 min
  17. Function and regulation of the PDK1 kinase

  • Prof. Dario Alessi
• 63 min
  18. LKB1 pathway and its role in cancer

  • Prof. Dario Alessi
• 51 min
  19. WNK1 pathway and its role in regulating hypertension

  • Prof. Dario Alessi
• 33 min
  20. The hyperphosphorylation of tau and Alzheimer's disease

  • Prof. Michel Goedert
• Protein Kinases as Targets for the Development of Anti-Cancer Drugs
• 68 min
  21. PI3K/AKT signaling in cancer

  • Prof. Neal Rosen
• 37 min
  22. RAS and RAF signaling in melanoma: biology and therapies

  • Prof. Richard Marais
• 38 min
  23. The mTOR kinase as a target for anti-cancer drugs

  • Prof. David Sabatini
• Archived Lectures *These may not cover the latest advances in the field
• 51 min
  24. Modular protein-protein interactions provide a general mechanism to organize dynamic cellular systems

  • Prof. Tony Pawson
• 38 min
  25. AMP-activated protein kinase: regulating cellular and whole body energy balance

  • Prof. Grahame Hardie

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Role of protein phosphatases
3. Classification of protein phosphatases
4. Classical protein tyrosine phosphatase family
5. PTP sequence motif
6. Protein tyrosine phosphatase 1B
7. Protein tyrosine phosphatase 1B - 3D structure
8. PTB1B - peptide complex
9. Catalytic site of PTP1B
10. PTP loop structure
11. PTP1B reaction mechanism
12. Activation segment of IRK
13. PTP1B-IRK peptide complex (1)
14. PTP1B-IRK peptide complex (2)
15. Redox regulation of protein tyrosine phosphatases
16. Oxidation states of cysteine
17. PTP loop of oxidised PTP1B (1)
18. PTP loop of oxidised PTP1B (2)
19. Oxidation induced conformational changes
20. Time course of PTP1B oxidation
21. SH2 domain PTPs: SHP2
22. Receptor-like PTPs: LAR
23. PTP superfamily structure comparison
24. CDK2-KAP complex
25. Eyes absent (Eya) protein tyrosine phosphatase
26. Protein Ser/Thr phosphatases (PPP) family
27. Protein phosphatase 1
28. PPP (PP1) reaction mechanism
29. PP1 surface representation
30. Protein phosphatase 1 - inhibitor complexes
31. RVxF motif
32. PP1 - MYPT1 complex
33. Protein phosphatase 2A
34. HEAT repeat sequences of PR65/A-subunit
35. HEAT repeats of PR65/A subunit
36. Protein phosphatase 2A holoenzyme
37. Auto-inhibited protein phosphatase 2B
38. PP2B/calcineurin-cyclosporin/cyclophilin complex
39. Protein phosphatase 5
40. TPR domain intra-repeat
41. Protein Ser/Thr phosphatases (PPM) family
42. Protein phosphatase 2C
43. Summary
44. Further reading

Topics Covered

• Reversible protein phosphorylation
• Classification of protein phosphatases
• Protein tyrosine phosphatase family, structure of PTP1B, catalytic mechanism, basis for pTyr specificity, recognition of IRK, regulation by ROS and cyclic sulphenamide
• Dual specificity phosphatases
• Eya protein tyrosine phosphatases
• PPP family of Ser/Thr phosphatases, structure of PP1, catalytic mechanism, inhibition by okadaic acid and microcystin, targeting subunits, PP2A, PP2B, PP5
• PPM family of Ser/Thr phosphatases, structure, catalytic mechanism
• Summary

Links

Series:

• Protein Phosphorylation

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on November 30, 2010

Financial Disclosures

• Prof. David Barford has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.