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1. Introduction
2. Role of protein phosphatases
3. Classification of protein phosphatases
4. Classical protein tyrosine phosphatase family
5. PTP sequence motif
6. Protein tyrosine phosphatase 1B
7. Protein tyrosine phosphatase 1B - 3D structure
8. PTB1B - peptide complex
9. Catalytic site of PTP1B
10. PTP loop structure
11. PTP1B reaction mechanism
12. Activation segment of IRK
13. PTP1B-IRK peptide complex (1)
14. PTP1B-IRK peptide complex (2)
15. Redox regulation of protein tyrosine phosphatases
16. Oxidation states of cysteine
17. PTP loop of oxidised PTP1B (1)
18. PTP loop of oxidised PTP1B (2)
19. Oxidation induced conformational changes
20. Time course of PTP1B oxidation
21. SH2 domain PTPs: SHP2
22. Receptor-like PTPs: LAR
23. PTP superfamily structure comparison
24. CDK2-KAP complex
25. Eyes absent (Eya) protein tyrosine phosphatase
26. Protein Ser/Thr phosphatases (PPP) family
27. Protein phosphatase 1
28. PPP (PP1) reaction mechanism
29. PP1 surface representation
30. Protein phosphatase 1 - inhibitor complexes
31. RVxF motif
32. PP1 - MYPT1 complex
33. Protein phosphatase 2A
34. HEAT repeat sequences of PR65/A-subunit
35. HEAT repeats of PR65/A subunit
36. Protein phosphatase 2A holoenzyme
37. Auto-inhibited protein phosphatase 2B
38. PP2B/calcineurin-cyclosporin/cyclophilin complex
39. Protein phosphatase 5
40. TPR domain intra-repeat
41. Protein Ser/Thr phosphatases (PPM) family
42. Protein phosphatase 2C
43. Summary
44. Further reading

Topics Covered

• Reversible protein phosphorylation
• Classification of protein phosphatases
• Protein tyrosine phosphatase family, structure of PTP1B, catalytic mechanism, basis for pTyr specificity, recognition of IRK, regulation by ROS and cyclic sulphenamide
• Dual specificity phosphatases
• Eya protein tyrosine phosphatases
• PPP family of Ser/Thr phosphatases, structure of PP1, catalytic mechanism, inhibition by okadaic acid and microcystin, targeting subunits, PP2A, PP2B, PP5
• PPM family of Ser/Thr phosphatases, structure, catalytic mechanism
• Summary

Links

Series:

• Protein Phosphorylation

Categories:

• Biochemistry

Talk Citation

Barford, D. (2010, November 30). Structure and mechanisms of protein phosphatases [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/VONR2422.
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Publication History

• Published on November 30, 2010

Financial Disclosures

• Prof. David Barford has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.