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1. Introduction
2. NK cells
3. The steps of NK cell-mediated cytotoxicity
4. ADCC, reverse ADCC and natural cytotoxicity
5. NK cell activating receptors
6. Role of the protein tyrosine kinase
7. Activating receptors on NK cells
8. Part I: ITAM-containing signaling adaptor proteins
9. Activation of Syk family kinases
10. Role of Syk in human NK cells
11. Role of Syk in mouse NK cells
12. Syk, ZAP70 and ITAM dependent receptors
13. Downstream targets regulating NK cytotoxicity
14. PI3 kinase recruitment and activation by SyK
15. Syk regulates PI3K
16. PI3K activity is required for NK cell activity
17. PI3K regulates MAPK activity
18. Activation of Rac leads to MAPK activation
19. PAK1 is involved in MAPK activation
20. The LAT adaptor protein
21. LAT is required for PLC-gamma-1 phosphorylation
22. LAT/NTAL and DAP12-initiated cytotoxicity
23. PLC-gamma-1and 2 produce IP3 and DAG
24. NK target cells induce a rise in intracellular Ca
25. PLC-gamma-2 is required for NK cell killing in vivo
26. PLC-gamma-2 is not needed for MTOC polarization
27. Vav1
28. Phosphorylated Vav1 increases NK cell killing
29. Rho family GTP-binding proteins
30. Summary of part I

Topics Covered

• NK cell cytotoxicity
• Introduction to NK cell activating receptors
• Regulation of NK cell cytotoxicity by immunoreceptor tyrosine-based activation motif associated receptors
• The downstream targets regulating NK cytolytic activity
• PI3K pathway is required for the development of cellular cytotoxicity
• LAT/NTAL are required for DAP12-initiated cytotoxicity and production of interferon gamma
• PLCγ2 is required for NK cell killing
• Vav proteins are linked to NK cell activating receptors and regulate the development of cellular cytotoxicity
• Rho family GTP-binding proteins regulate NK cell-mediated cytotoxicity

Links

Series:

• Natural Killer Cell Biology

Categories:

• Biochemistry
• Cell Biology
• Immunology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Publication History

• Published on October 20, 2010

Financial Disclosures

• Prof. Dan Billadeau has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.