• Introduction
• 38 min
  1. DNA methylation during development

  • Prof. Howard Cedar
• The Nuts and Bolts of DNA Methylation
• 54 min
  2. DNA methylation patterns in mammals

  • Prof. John Greally
• 31 min
  3. Proteins that bind methylated DNA

  • Dr. Pierre-Antoine Defossez
• DNA Methylation and Normal Physiology
• 25 min
  4. Genomic imprinting

  • Prof. Marisa Bartolomei
• 32 min
  5. DNA methylation and stem cells

  • Dr. Alexander Meissner
• DNA Methylation and the Pathophysiology of Disease
• 54 min
  6. DNA methylation changes in aging cells and tissues

  • Prof. Jean-Pierre Issa
• 61 min
  7. DNA methylation in cancer

  • Prof. Paula Vertino
• 29 min
  8. Epigenomics of complex disease

  • Prof. Art Petronis
• 41 min
  9. DNA methylation, inflammation and cancer

  • Prof. Gerd Pfeifer
• Clinical Applications of DNA Methylation Research
• 41 min
  10. DNA methylation as a tumor marker: implications for diagnosis, early detection and risk assessment

  • Prof. Steve Belinsky
• 46 min
  11. Reversing DNA methylation pharmacologically

  • Dr. Allen Yang

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Talk overview
3. A few introductory notions
4. Reminder and graphic convention
5. Methylated DNA in 3D
6. The number of methylated CpGs per genome
7. The distribution of methylated CpGs in cells
8. DNA methylation and cancer
9. The identification of methyl-binding proteins (MBPs)
10. Why look for MBPs in the first place?
11. Biochemical identification of MBPs
12. Finding other MBPs by homology searches
13. Methyl binding proteins: Gel-retardation assay
14. Methyl binding proteins: Nuclear localization (1)
15. Methyl binding proteins: Nuclear localization (2)
16. Methyl binding proteins: Chromatin-IP
17. Human methyl-binding proteins list
18. What do the methyl-binding proteins do?
19. MBPs cause heterochromatinization
20. MeCP2, Rett syndrome, and neuronal transcription
21. Search for MeCP2 target genes in the mouse brain
22. MBD4, cancer, and mutagenesis
23. Spontaneous base deamination
24. UHRF1 is necessary for embryonic viability
25. UHRF1 and the maintenance of DNA methylation
26. Kaiso: a bifunctional transcription factor
27. Kaiso in colon cancer
28. Unresolved questions
29. Why are most MBPs non-essential?
30. Proteomics yield many new MBP candidates
31. New MBPs might be identified by genetic screens
32. The role of MBPs in active DNA demethylation?
33. Summary
34. Identification of methyl-binding proteins
35. Three families of methyl-binding proteins
36. General model
37. Acknowledgements
38. Reading suggestions
39. References

Topics Covered

• Introductory notions: distribution, abundance, and consequences of DNA methylation
• The methyl-binding proteins: who they are, how they were found
• What do the methyl-binding proteins do? Four examples
• Unresolved questions and future perspectives
• References

Links

Series:

• DNA Methylation

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on March 3, 2011

Financial Disclosures

• Dr. Pierre-Antoine Defossez has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.