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Navigable Slide Index

1. Introduction
2. The actions of BiP
3. Overview
4. BiP's effects on CIA
5. Protocol for CIA disease studies
6. IV BiP prevents CIA in mice
7. BiP suppresses active CIA in mice
8. Therapeutic use of BiP
9. BiP's special properties
10. Adoptive transfer BiP specific cells
11. Adoptive transfer: prevention and therapy
12. Mechanism of the therapeutic effect
13. Soluble BiP primes TH2 cells
14. Cytokine production at end of therapy
15. BiP fails to suppress active arthritis
16. Therapy of arthritis requires IL-4
17. Summary effect BiP on CIA
18. BiP-specific T-cell clones
19. Proliferation of BiP responsive clones
20. IL-10 production by BiP responsive clones
21. Cytokine production of BiP responsive clones
22. BiP specific T cell clones - conclusions
23. Alternate macrophage activation
24. Monocyte differentiation
25. Cytokines
26. Cytokine receptors
27. Costimulatory molecules
28. Osteoclast development and function
29. Osteoclast development - experimental protocol
30. Inhibition of osteoclast differentiation
31. BiP's effect on F-actin rings
32. Inhibition of resorption
33. Murine calvarial model
34. BiP inhibits resorption in MCM
35. SCID-RA SM chimeras
36. Generation of SCID-RA SM chimeras
37. Weight of implanted tissues
38. Histopathological features
39. Immuno-histochemical staining: CD86
40. Immuno-histochemical staining: HLA-DR
41. Immuno-histochemical staining: TNF-alpha
42. Production of cytokines in mouse serum
43. Summary
44. Conclusion
45. Acknowledgements

Topics Covered

• BiP is a molecular chaperone found within the endoplasmic reticulum
• It protects cells from damage and death by stress
• It can also appear outside the cell when it engages an as yet undefined cell membrane receptor, which alters monocyte differentiation and function
• BiP can prevent and treat collagen induced arthritis by a single intravenous or a single subcutaneous injection of BiP
• This therapeutic effect appears to be linked at least in part to the production of interleukin 4
• Lymph node and spleen cells from mice injected with BiP can prevent and treat ongoing arthritis when transferred intravenously into recipient mice without the necessity of additional BiP having to be given
• BiP given intravenously to severe combined immunodeficiency mice bearing subcutaneous, vascularised implants of rheumatoid arthritis synovial membrane will ablate inflammatory cells and cytokines from the implants

Links

Categories:

• Biochemistry
• Cell Biology
• Immunology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Panayi, G. (2010, March 31). Treatment of rheumatoid arthritis by the induction of regulatory cells: BiP is a promising candidate [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 4, 2024, from https://doi.org/10.69645/DHYH1115.
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Publication History

• Published on March 31, 2010

Financial Disclosures

• Prof. Gabriel Panayi has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.