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1. Introduction
2. Nucleo-mitochondrial interactions
3. Nuclear and mitochondrial respiratory subunits
4. Mammalian somatic cytochrome c genes
5. Identification of NRF-1
6. NRF-1 functional domains
7. NRF-2, an activator of COXIV expression
8. NRF-2 functional domains
9. NRF-2 binding sites
10. Comparison of NRF target genes
11. Partial list of NRF target genes
12. Promoter occupancy by NRF-2
13. NRF-1 knockout allele
14. NRF-1 genotypes
15. Properties of NRF-1(-/-) blastocysts
16. Mitochondrial transcription factors
17. Transcription factor promoters
18. Comparison of TFB NRF-1 sites
19. Alignment of TFB NRF-2 sites
20. NRF control over TFB promoters
21. NRF control of mitochondrial transcription
22. Transcription factors and bioenergetic phenotype
23. Transcriptional co-activators
24. Functional domains in PGC-1-alpha (92 kDa)
25. Metabolic regulation by PGC-1-alpha
26. PGC-1-related co-activator (PRC)
27. Activator/coactivator interactions
28. PRC functional interaction with NRF-1
29. Trans-activation of TFB expression
30. Coactivator induction during thermogenesis
31. PRC expression and cell growth
32. Protein synthesis and PRC mRNA expression
33. Immediate early expression of PRC
34. Serum-induced gene expression
35. Respiratory response to serum
36. Pathways of CREB signaling
37. Binding of NRF-1 and CREB to PRC
38. NRF-1/PRC and CREB/PRC complexes
39. Summary of in vitro binding
40. Quantitative ChIP analysis
41. Cytochrome c promoter occupancy
42. Model for growth induction
43. Integrative role for PRC in cell growth
44. Coactivator control of cell metabolism

Topics Covered

• Contribution of nuclear and mitochondrial genomes to respiratory chain expression
• Genomic origin of respiratory chain subunits
• Cytochrome c as a model for respiratory gene expression
• DNA binding and transcriptional properties of nuclear respiratory factors (NRF-1 and NRF-2)
• Identification and characterization of NRF target genes
• Phenotypes associated with a targeted disruption of NRF-1 in mice
• NRF control of mitochondrial transcription factor expression
• The PGC-1 family
• PRC functional interactions with NRF-1 and CREB
• Cytochrome c promoter occupancy by PRC during the transition from quiescence to proliferative growth
• Potential role for PRC in integrating mitochondrial functions with cell growth

Links

Series:

• Mitochondrial Biogenesis

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Scarpulla, R. (2007, October 1). Nuclear control of respiratory chain expression by transcriptional activators and coactivators [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved January 21, 2025, from https://doi.org/10.69645/CWNJ6308.
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Publication History

• Published on October 1, 2007
• Archived on June 30, 2019

Financial Disclosures

• Prof. Richard Scarpulla has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.