Registration for a live webinar on 'Precision medicine treatment for anticancer drug resistance' is now open.
See webinar detailsWe noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
Printable Handouts
Navigable Slide Index
- Introduction
- Antibiotic susceptibility testing
- Mortality vs. therapy
- The importance of susceptibility testing
- Rationale for antibiotic susceptibility testing
- Test methods
- DISC susceptibility test
- DISC susceptibility test - an example
- MIC determination
- MIC determination by broth or agar dilution
- MIC determination by broth dilution
- MIC determination by agar dilution
- Factors affecting zone size
- Factors affecting MICs
- Standardizing susceptibility testing methods
- Standardizing testing in England
- Journal of antimicrobial chemotherapy 2009
- BSAC standardized testing method
- BSAC standardized disc testing method (1)
- BSAC standardized disc testing method (2)
- Template for use with BSAC
- BSAC vs. CLSI
- Correlation of zone size and MIC
- E-test for MIC determination (1)
- E-test for MIC determination (2)
- E-test for testing multiple antibiotics
- E-test strips for testing ESBL production
- Interpretation of susceptibility tests
- Defining susceptibility and resistance
- EUCAST
- EUCAST MIC breakpoints
- Clinical breakpoints
- Microbiological breakpoints
- Ciprofloxacin MICs vs. E. coli
- Establishing breakpoints
- Ciprofloxacin - EUCAST MIC breakpoints
- Penicillin - EUCAST MIC breakpoints
- EUCAST breakpoints database
- EUCAST rationales: published in CMI too
- EUCAST vs. CLSI breakpoints
- Automating antibiotic susceptibility testing
- Vitek 2 AST system (1)
- Vitek 2 AST system (2)
- Advanced expert system (AES)
- Interpretive reading - examples
- Interpretive reading - limits
- Pros and cons of automated testing
- The future of susceptibility testing
Topics Covered
- Antibiotic susceptibility testing: rationale
- Disc susceptibility test
- Determination of MICs
- Broth vs. agar dilution
- Standardizing methods
- BSAC standardized testing method
- BSAC vs. CLSI
- MIC determination by E-test
- MICs of multiple antibiotics for the same bacterial strain
- Testing E. coli
- Interpretation of susceptibility tests
- Defining susceptibility and resistance
- European Committee on Antimicrobial Susceptibility testing (EUCAST)
- EUCAST MIC breakpoints
- Clinical breakpoints
- Microbiological breakpoints
- Establishing breakpoints
- Automating antibiotic susceptibility testing
- Vitek 2 AST system
- Advanced expert system
- Interpretive reading: the limits
- The future
Links
Series:
Categories:
Therapeutic Areas:
Talk Citation
Johnson, A. (2010, January 13). Conventional and automated diagnostic methods [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 14, 2024, from https://doi.org/10.69645/WPHQ9646.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Alan Johnson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
A selection of talks on Clinical Practice
Transcript
Please wait while the transcript is being prepared...
0:00
I'm Dr. Alan Johnson.
In this presentation, I'm
going to give an overview of
conventional and
automated methods of
anti-microbial susceptibility
testing methods,
commonly used in clinical
microbiology laboratories.
0:14
It is well documented
that patients
with serious
bacterial infections
who are treated with
antibiotics to which
the infecting pathogen
is not susceptible,
have poorer clinical outcomes
manifest by increased
morbidity and mortality.
In addition, such
patients frequently
have prolonged
lengths of hospital
stay with associated increase
in the costs of their
health care provision.
0:38
This slide shows a number
of such studies with
the percentage
mortality listed on
the horizontal axis,
and that you will see,
a reproducible finding from
all of the studies was,
in those patients who received
inappropriate therapy,
the degree of mortality
was statistically
significantly higher
than in those patients for whom
the therapy was appropriate.
1:00
Thus, anti-microbial
susceptibility testing is
an important part
of the management
of patients with infections.
At the level of the
individual patient,
results of susceptibility
testing can be
used to target and
guide the treatment.
At a general level
the accumulation of
susceptibility testing data in
individual laboratories
means that it is
possible to come up with
empirical guidelines for
the treatment of
further patients
based on the known
likely pathogens,
and their known levels
of resistance or
susceptibility to particular
antimicrobial agents.
1:31
The underlying rationale for
the laboratory testing of
antimicrobial agents is
to expose bacteria to
these antibiotics under
defined laboratory conditions,
and to determine
what effect, if any,
the presence of the
antibiotic has on
the growth and survival
of the organism.