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1. Introduction
2. Staphylococcus aureus
3. The envelope of gram-positive bacteria
4. Protein A
5. Sortase A - anchoring proteins
6. S. aureus sortase A mutants
7. Sortase A cleavage of LPXTG peptides
8. Sortase A catalyzed transpeptidation
9. LPXTG peptide bound to sortase A
10. Deposition of protein A in the envelope
11. Protein A on the staphylococcal surface
12. Signal peptides of staphylococcal surface proteins
13. Distribution of surface proteins in cell wall envelope
14. SPs address proteins to specific locations
15. Protein traffic to the cell wall envelope of S. aureus
16. Fate of S. aureus injected into mice bloodstream
17. Abscess formation in the kidney of mice
18. Architecture of day 2 lesions
19. Architecture of day 5 lesions
20. SEM of staphylococci in host tissues
21. Sortase mutants cannot form abscesses
22. Sortase A is essential for persistence in host
23. Sortase mutants in renal tissue
24. Surface abscesses in wt and srtA mutants
25. Surface proteins involved in abscess formation
26. What are the protective antigens of staphylococci?
27. Surface proteins and protective immunity
28. Vaccine against abscess with IsdAB/SdrDE
29. Staphylococcal abscesses enable persistence
30. Heme-iron transport in staphylococci
31. Sortase B and the IsdC anchor structure
32. Protein topologies in the cell wall of staphylococci
33. Staphylococcal envelope associated proteins
34. Eap and Emp
35. Lack of envelope associated proteins
36. Eap mutants form abscesses
37. Emp mutants cannot form abscesses
38. Distribution of Eap, Emp in abscess formation
39. Eap and Emp vaccines
40. Vaccine protection against lethal challenge
41. S. aureus and human lung infections
42. Staphylococcus aureus pneumonia in mice
43. Murine pneumonia model
44. Mechanisms of Hla assembly and function
45. Hla is an essential virulence factor for S. aureus
46. Active immunization with Hla
47. Passive immunization with Hla antibodies
48. Summary and acknowledgments

Topics Covered

• The envelope of gram-positive bacteria
• Anchoring proteins
• Signal peptides of staphylococcal surface proteins
• Protein traffic to the cell wall envelope of S. aureus
• Fate of S. aureus injected into mice bloodstream
• Abscess formation
• What are the protective antigens of staphylococci?
• Surface proteins and protective immunity
• Staphylococcal abscesses enable persistence
• Protein topologies in the cell wall of staphylococci
• Vaccine protection against lethal challenge
• S. aureus and human lung infections
• Active/passive immunization

Links

Series:

• Molecular Basis of Bacterial Infection

Categories:

• Clinical Practice
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Schneewind, O. (2009, November 2). Molecular pathogenesis and prevention of Staphylococcus aureus infections [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/VAKP3485.
Export Citation (RIS)

Publication History

• Published on November 2, 2009

Financial Disclosures

• Prof. Olaf Schneewind has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.