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Printable Handouts
Navigable Slide Index
- Introduction
- Recognition of bacteria by airway epithelial cells
- A cystic fibrosis patient's airway
- Questions that need to be addressed
- Epithelial cells express toll like receptors
- Signaling through the TLR2
- Characteristics of airway epithelial cells
- Epithelial cells' response to presence of bacteria
- Bacteria stimulate mobilization of receptors
- Questions of interest
- Calcium as a second messenger
- How is the Calcium (Ca2+) released in the cell?
- TLR2 stimulation mediates the release of Ca2+
- Bacterial Ca2+ fluxes induction is TLR2 dependent
- Ca2+ release signals proinflammatory responses
- Bacterial induction of NF-kB is TLR2 dependent
- TLR2 stimulation signals Ca2+ fluxes
- PI3K is required for TLR2 mediated Ca2+ fluxes
- PLC-G is required for TLR2 mediated Ca2+ fluxes
- Summary of the process described so far
- Junctional components of epithelial cells
- Does Ca2+ act as an inter-cellular messenger?
- Connexins and gap junctions
- Connexin's structural organization
- Signaling moves from cell to cell via gap junctions
- Gap junction inhibition attenuates IL-8 secretion
- Do TLR2 Ca2+ fluxes travel through gap junctions?
- Airway cells communicate TLR2 signals via GJ
- Is GJ communication biologically significant?
- What is a possible mechanism for this regulation?
- Cell communication is decreased after stimulation
- Calcium involvement in epithelial signaling
- P. aeruginosa modifies epithelial permeability
- Occludin, E-cadherin altered in response to PAO1
- PAO1 (heat killed) does not alter barrier properties
- Epithelial junctions
- Occludin and E-cadherin
- Epithelia signaling modulates junction properties
- Does calpain target junctional proteins?
- Calpain is a Ca2+ dependent protease
- Calpain directly targets specific junctional proteins
- Calpain activation in airway epithelial cells
- TLR2 dependent calpain activation
- Calpain association with Occludin
- Occludin is cleaved by calpain
- Decrease in hyperphosphorylated Occludin
- Where is Occludin cleaved?
- Calpain association with E-cadherin
- Does calpain change epithelial junction properties?
- Calpain and PMN transepithelial migration
- Calpain activity contributes to inflammation in vivo
- Does calpain help recruit PMN to the airways?
- The proposed model
- Calpain summary
- Answers to the questions
- Acknowledgements
Topics Covered
- Inhaled bacteria activate Ca2+ fluxes in airway cells
- Signal proinflammatory chemokine and cytokine production
- Ca2+ acts as a second messenger to stimulate adjacent cells
- Ca2+ activates proteases to facilitate PMN transmigration
Links
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Talk Citation
Prince, A. (2009, October 29). Bacterial infection of epithelial signaling [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 14, 2024, from https://doi.org/10.69645/LEDL3870.Export Citation (RIS)
Publication History
Financial Disclosures
- There are no financial matters to disclose.