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Biomedical Basics

Gene cloning and recombinant DNA

  • Created by Henry Stewart Talks
Published on December 31, 2025   4 min

A selection of talks on Infectious Diseases

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In this talk, we turn our attention to gene cloning and recombinant DNA framing our discussion around the core concepts and laboratory processes of gene cloning and recombinant DNA, including gene isolation, vector selection and tools like restriction enzymes and DNA ligase. We will examine how recombinant DNA is introduced into host organisms enabling protein production for diverse applications in medicine and industry. The lecture will highlight major breakthroughs such as the creation of genetically modified organisms and therapeutic proteins as well as address ethical and safety considerations that arise from these transformative technologies. We will explore the key principles and processes that underpin much of modern biology, biotechnology and medicine. Gene cloning enables precise copying and manipulation of DNA segments usually genes by inserting them into host organisms often bacteria. We will discuss how genes are isolated, cloned and expressed, the biological tools involved, major applications in medicine and agriculture and the ethical considerations of this technology. Central to gene cloning is isolating the gene of interest from cellular DNA. This can begin with messenger RNA using reverse transcription to generate complementary DNA or directly from genomic DNA via polymerase chain reaction. The isolated gene is then inserted into a cloning vector, a DNA molecule that replicates in a host. Common vectors include plasmids,

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Gene cloning and recombinant DNA

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