• Co-ordination of G1 Progression
• 37 min
  1. START control in yeast

  • Prof. Curt Wittenberg
• 33 min
  2. The pRB/E2F pathway

  • Prof. Jacqueline Lees
• 35 min
  3. Cell cycle control by the ubiquitin system in mammals

  • Prof. Michele Pagano
• Chromosome Duplication
• 47 min
  4. Replication licensing

  • Prof. Julian Blow
• 51 min
  5. Initiation of DNA replication

  • Prof. Bruce Stillman
• 48 min
  6. Regulation of replication fork progression and stability

  • Dr. Luis Aragón
• 35 min
  7. Nucleosome assembly during DNA replication

  • Dr. Alain Verreault
• Preparing for Mitosis
• 27 min
  8. Sister chromatid cohesion: simple concept, complex reality

  • Prof. Douglas Koshland
• 50 min
  9. Mitotic chromosome condensation

  • Prof. Andrew Belmont
• 33 min
  10. Centrosome duplication and separation in animal cells

  • Prof. Andrew Fry
• Spindle Assembly and Chromosome Segregation
• 30 min
  11. Bipolar spindle assembly

  • Dr. Eric Karsenti
• 54 min
  12. Chromosome biorientation in yeast

  • Prof. Mike Stark
• 43 min
  13. Regulation of mammalian cell division by the chromosomal passenger complex

  • Dr. Susanne Lens
• Mitotic Exit and Cytokinesis
• 39 min
  14. Cleavage furrow formation and ingression during animal cytokinesis

  • Dr. Pier Paolo D'Avino
• Checkpoints Governing Cell Cycle Progression
• 34 min
  15. The DNA damage response

  • Dr. Vincenzo Costanzo
• 54 min
  16. The spindle checkpoint

  • Dr. Kevin Hardwick
• 27 min
  17. Spindle movement and checkpoint control during mitosis in yeast

  • Prof. John Cooper
• 43 min
  18. The G2/M transition

  • Prof. Dr. René Medema
• The Cell Cycle in Development and Cancer
• 62 min
  19. Mouse models to investigate cell cycle and cancer

  • Dr. Philipp Kaldis
• 18 min
  20. Cell cycle: a complex network of signals regulating cell proliferation

  • Prof. Antonio Giordano
• 40 min
  21. Drug discovery and target validation in the p53 pathway

  • Prof. Sir David Lane
• 30 min
  22. Role and regulation of Cdk inhibitors in development and cancer

  • Prof. Martine Roussel
• 48 min
  23. The kinetochore as a target for the development of mitosis specific anti-cancer drugs

  • Dr. Tim Yen
• 47 min
  24. The Myc transcription factor network

  • Prof. Robert N. Eisenman
• Meiosis: A Specialized Cell Cycle
• 58 min
  25. Recombination and the formation of chiasmata in meiosis

  • Prof. Matthew Whitby
• Archived Lectures *These may not cover the latest advances in the field
• 24 min
  26. Geometric regulation of kinetochore orientation

  • Prof. Yoshinori Watanabe

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Talk outline
3. What is chromosome bi-orientation?
4. A model of chromosome bi-orientation
5. Chromosome bi-orientation and cell division
6. Cohesin role during chromosome bi-orientation
7. Sister chromatid attachment to microtubules
8. The budding yeast as a model for bi-orientation
9. Monitoring sister centromeres in yeast
10. Metaphase-arrested cell
11. Bi-orientation leads to tension
12. Special structure of chromatid under tension
13. Kinetochore capture - a necessary pre-requisite
14. Kinetochore capture in yeast
15. Kinetochore capture: yeast vs. metazoans
16. Achieving chromosome bi-orientation
17. Geometry-dependent mechanism?
18. Correction mechanism?
19. Yeast Ipl1p kinase
20. Chromosomes fail to bi-orient in ipl1 mutants
21. Role of Ipl1 in chromosome bi-orientation (1)
22. Role of Ipl1 in chromosome bi-orientation (2)
23. How might Ipl1p kinase promote bi-orientation?
24. Bi-orientation without back-to-back geometry (1)
25. Bi-orientation without back-to-back geometry (2)
26. Conclusions from mini-chromosomes studies
27. Ipl1p - substrates and mechanisms
28. The yeast chromosomal passenger complex
29. Models for tension-sensing
30. CPC and kinetochores in MCD1 mutant
31. Targets of Ipl1p kinase at the kinetochore
32. Structure of the yeast kinetochore
33. The Ndc80 complex
34. Ipl1p phosphorylates Ndc80
35. DASH/Dam1p complex
36. Dam1 alignment - S.cerevisiae vs. S. castellii
37. Mutations in Dam1 and their consequences
38. Model for Ipl1 function in promoting bi-orientation
39. Targets of Aurora B in other organisms
40. Other components required for bi-orientation
41. Ipl1p, bi-orientation and spindle checkpoint
42. Conclusions

Topics Covered

• What is chromosome biorientation and why is it important?
• Failure to bi-orient a chromosome will lead to mis-segregation/aneuploidy
• Modes of sister chromatid attachment to spindle microtubules
• The budding yeast (Saccharomyces cerevisiae) as a model for studying chromosome bi-orientation
• Monitoring sister centromeres on individual yeast chromosomes
• Kinetochore capture
• Achieving chromosome bi-orientation: mechanisms
• Geometry-dependent mechanism or a correction mechanism?
• Yeast Ipl1p kinase
• Chromosomes fail to bi-orient with high frequency in Ipl1 mutants
• How might Ipl1p kinase promote bi-orientation?
• Yeast chromosomes can bi-orient without the need for back-to-back geometry
• How does Ipl1p kinase promote bi-orientation?
• The yeast chromosomal passenger complex
• Models for tension-sensing
• What are the targets of Ipl1p kinase at the kinetochore?
• Model for Ipl1 / Aurora function in promoting bi-orientation
• Does Aurora B have similar targets in other organisms?
• Other components required for bi-orientation
• Ipl1p kinase may coordinate chromosome bi-orientation and spindle checkpoint activation

Links

Series:

• The Cell Division Cycle

Categories:

• Cell Biology
• Microbiology

Therapeutic Areas:

• Oncology

Talk Citation

Publication History

• Published on May 6, 2009

Financial Disclosures

• Prof. Mike Stark has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.