• The Basics of Microarrays
• 42 min
  1. How to design a successful microarray experiment

  • Dr. Rainer Breitling
• Microarrays in the Understanding of the Biology of the Cell and Whole Organism
• 51 min
  2. Genome-wide mapping of estrogen: receptor binding sites and activity

  • Prof. Jason Carroll
• Microarrays in the Diagnosis, Prognosis and Treatment of Disease
• 35 min
  3. Single nucleotide polymorphism microarrays in the analysis of cancer

  • Dr. Rameen Beroukhim
• Archived Lectures *These may not cover the latest advances in the field
• 23 min
  4. The use of statistics in microarray studies

  • Dr. Ernst Wit
• 42 min
  5. Simultaneous microarray measurement of nuclear and whole cell changes in gene expression reveals overlapping but distinct patterns of transcriptional regulation

  • Dr. Chris Cheadle
• 38 min
  6. Global analysis of gene translation

  • Prof. Philip Tofilon
• 36 min
  7. Microarrays in the study of the basic mechanisms of evolution

  • Dr. Greg Gibson
• 41 min
  8. The use of microarrays to understand the biology and toxicology of low dose arsenic

  • Prof. Joshua Hamilton
• 46 min
  9. The bioinformatics of microarrays

  • Dr. Maureen Sartor
• 44 min
  10. Genome-wide profiling of microRNAs: from scientist benchside to patient bedside

  • Prof. George Calin
• 42 min
  11. Microarrays: their design and use

  • Prof. Craig R. Tomlinson
• 67 min
  12. Cancer systems biology: dissection and analysis of context-specific molecular interaction networks

  • Prof. Andrea Califano
• 35 min
  13. Utilizing microarrays to study posttranscriptional regulatory mechanisms of plants

  • Dr. Brian Gregory

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Overview
3. Toxic metals in the environment
4. Arsenic as an environmental contaminant
5. Arsenic as a causative agent in disease
6. Arsenic contamination in New England
7. Arsenic microarray - one-color
8. Effects of metals on gene expression
9. Effects of arsenic on gene expression
10. Daphnia pulex microarrays as a model
11. Analysis of Daphnia pulex results
12. Arsenic effects on Daphnia genes
13. Comparison of SAM and QED analysis
14. Non-parametric gene expression
15. Rank product analysis of microarrays
16. Parametric method limitations
17. Long gene lists - how long?
18. Individual genes vs. patterns
19. Canonical pathway analysis
20. Non-canonical networks of genes
21. Arsenic effects in mouse lung
22. Arsenic effects in lung - doses
23. Arsenic effects in lung - heat map
24. Arsenic effects in cells - doses
25. Phenotypic switching by arsenic
26. Effects of diet on gene expression
27. Arsenic as an Endocrine Disruptor
28. Arsenic disruption of GR in vivo
29. Arsenic effects on IL1B network
30. Arsenic effects on TLR signaling
31. Summary of statistical methods
32. Summary of arsenic effects
33. Acknowledgements

Topics Covered

• Arsenic as an environmental chemical of concern for human health
• Use of microarrays to understand patterns of gene expression changes in response to low levels of toxic metals
• Development of non-parametric techniques for evaluating results of microarrays
• Use of patterns and biological pathways to understand microarray results
• Microarray analysis of low dose arsenic effects

Links

Series:

• Microarrays

Categories:

• Biochemistry
• Cell Biology
• Methods

Talk Citation

Publication History

• Published on March 30, 2009
• Archived on February 25, 2021

Financial Disclosures

• Prof. Joshua Hamilton has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.