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1. Epigenetics - Definition
2. Epigenetic marks
3. Marking DNA by cytosine methylation
4. Cytosine methylation evolutionarily volatile
5. DNA methylation patterns
6. Pattern of mammalian DNA methylation
7. Questions of patterning
8. Maintenance of genomic CpG methylation patterns
9. De novo methylation of CpG islands
10. De-methylation of the paternal pronucleus
11. De-methylation of interleukin-2 promoter in T helper
12. What determines the pattern ?
13. CpGs in mammalian transposons
14. Ciona intestinalis/Ascidian larva
15. Stable mosaic of meth./non meth. domains (A)
16. Stable mosaic of meth./non meth. domains (B)
17. Non-methylated transposable elements
18. Immunity to DNA methylation
19. What triggers DNA methylation?
20. The consequences of DNA methylation
21. Deamination of cystocine & 5-methylcytosine
22. 5-Methylcytosine is an endogenous mutagen
23. Reading the methylation signal (A)
24. Reading the methylation signal (B)
25. Methyl-CpG binding protein family
26. MBD2 - the ancestral MBD protein
27. MeCP1: detectable in crude nuclear extracts
28. MeCP1 = MBD2 & NuRD complex
29. Biological role of MBD3
30. Differentiation of nave T helper cells
31. De-repression of interleukin-4 & interferon-g
32. In the absence of Mbd2:
33. Gene slicing depends on cooperation (A)
34. Gene slicing depends on cooperation (B)
35. DNA methylation & disease - What can go wrong? (A)
36. DNA methylation & disease-What can go wrong? (B)
37. Methyl-CpG binding protein family
38. Rett Syndrome
39. Rett Syndrome mutations in the MECP2 gene
40. MBD of MeCP2 docked with methylated DNA
41. Murine Bdnf gene modulated by MeCP3
42. Murine Bdnf gene modulated by MeCP2 (B)
43. Conclusions

Topics Covered

• DNA methylation as an epigenetic mark
• location and patterns of methylated and nonmethylated CpG sites in the vertebrate genome
• factors determining DNA methylation patterns
• association of DNA methylation with stable gene silencing, e.g. methylation of CpG islands on the inactive X-chromosome, ensures long term repression of associated genes
• recruitment of corepressors that impose a silent chromatin structure
• repulsion of transcriptional activators by methyl-CpG leading to gene silencing
• the link between human disorders, including cancer, Rett Syndrome and ICF syndrome, and defects in the DNA methylation system

Links

Series:

• Epigenetics

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on June 22, 2016

Financial Disclosures

• Prof. Adrian Bird has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.