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1. Introduction
2. Oxphos complex assembly
3. Outline: introduction and OXPHOS assembly
4. Outline: complexes IV, I and supercomplexes
5. Mitochondria: oval shaped organelles
6. Two membranes, 4 compartments
7. Mitochondria form a dynamic network
8. Mitochondria contain extra chromosomal DNA
9. Mitochondrial DNA
10. Main function of mitochondria: ATP production
11. Phosphorylation, e- transport and H+ generation
12. All components form together a functional complex
13. What are the OXPHOS complex components?
14. Nuclear and mitochondrial subunits
15. Co-evolution of mitochondrial and nuclear subunits
16. Additional components
17. How is the assembly coordinated
18. MtDNA replication, translation and regulation (1)
19. Over-expressing a single subunit (Cox4p)
20. Assembly
21. Which tools are required and what can go wrong?
22. MtDNA replication, translation and regulation (2)
23. Why study OXPHOS complex assembly?
24. How do we study assembly?
25. Two-dimensional blue-native PAGE
26. Complex IV (cytochrome c oxidase)
27. Cytochrome c oxidase assembly intermediates
28. Putative COX assembly pathway
29. The COX assembly is distributed in patients
30. Complex IV assembly
31. Complex I
32. Complex I assembly
33. Is complex I assembly a sequential process?
34. Is complex I a semi-sequential process?
35. Subunits and topology (1)
36. Complex I assembly in N. crassa
37. The modular evolution model
38. Conditional complex I assembly system creation
39. Dynamics of complex I subcomplexes
40. Composition of subcomplexes (1)
41. Composition of subcomplexes (2)
42. Subcomplexes of complex I
43. Complex I subunits presence per subcomplexes
44. Model for human complex I assembly
45. Subunits and topology (2)
46. Complex I crystal structure
47. Human complex I assembly
48. Complex I assembly distribution: deficient patients
49. Disturbed CI assembly in CI deficient patients
50. CI assembly proteins
51. Complex I chaperone NDUFAF1
52. Is NDUFAF1 localized in the mitochondrion?
53. Is NDUFAF1 involved in complex I assembly?
54. Involvement of assembly chaperones?
55. NDUFAF1 is a genuine CI assembly chaperone
56. Super complexes
57. Tracing the complexes on a gel
58. Respiratory complexes organised as respirosome
59. Some implications of supercomplexes
60. OXPHOS supercomplexes
61. The role of supercomplexes in cristae morphology
62. Supercomplexes: summary
63. Concluding remarks
64. Acknowledgments

Topics Covered

• The mitochondrial oxidative phosphorylation system
• Components: mitochondrial subunits, nuclear subunits and prosthetic groups
• Coordination of assembly
• Factors involved in assembly
• Defects in OXPHOS assembly
• Complex IV assembly
• Complex I assembly
• Complex I assembly defects
• Chaperones for complex I
• Supercomplexes of the OXPHOS system

Links

Series:

• Mitochondrial Biogenesis

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on October 28, 2018

Financial Disclosures

• Dr. Leo Nijtmans has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.