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Printable Handouts
Navigable Slide Index
- Introduction
- Why drugs?
- Why Drosophila?
- Ethanol - a non specific drug
- A quote by M. Ashburner
- Models of addiction
- The approach
- What do ethanol-exposed flies do?
- A really drunk fly
- The inebriometer
- Identification of important mutants and genes
- The genetic screen process (1)
- Cheapdate flies are sensitive in the inebriometer
- Absorption and metabolism of cheapdate flies
- Cheapdate is a mutation in amnesiac
- Amnesiac rescues cheapdate's ethanol sensitivity
- Drosophila mutants in cAMP signal transduction
- The unbearable likeness of beings
- Targeting NS disruptions
- Regulation of ethanol snsitivity by PKA activity
- GAL4/UAS system
- Genetic manipulation of PKA activity
- Reduced activity with PKA inhibitor expression
- Acute PKA inhibition increases ethanol sensitivity
- Use of a collection of GAL4 lines
- P[GAL4] x UAS-lacZ
- Brain expression of P[GAL4], line 201Y (1)
- MB ablation does not alter ethanol sensitivity
- Brain expression of P[GAL4], line 201Y (2)
- PI cells express insulin-like peptides (dILPs)
- Effect of PKA inhibition in IPCs
- PI neurons project to the ring gland
- The insulin receptor (INR) signaling pathway
- Reduced dINR function increases EtOH sensitivity
- Role of INR pathway in ethanol sensitivity
- PI3K inhibition increases EtOH sensitivity
- Expression of constituitivley active hFOXO
- The insulin receptor (INR) signaling pathway
- Insulin pathway and ethanol's rewarding effects
- Mechanisms in fly's responses to cocaine
- Psychostimulant exposure and behavioral analysis
- Mock exposure
- Acute cocaine exposure
- A simple behavioral assay
- Cocaine dose-response
- Mechanism of cocaine action
- Effect of dopamine depletion
- The genetic screen process (2)
- Effect of dLmo mutations on cocaine sensitivity
- Lmo genomic region
- How does dLMO work?
- Where does dLmo function?
- GAL4 expression in varios CNS patterns
- Mapping the dLmo site of action
- dLmo-GAL4 expression in PDF-positive cells
- What is known about LNvs?
- Does dLmo regulate cocaine sensitivity?
- Two possible models for dLmo and LNvs function
- Role of LNvs in cocaine sensitivity
- The Working model
- Summary
- The Drosophila model and human drug addiction
- Thank you
Topics Covered
- Behaviors induced by ethanol and cocaine in Drosophila
- Genes and pathways that regulate ethanol-induced behaviors: amnesiac, and the cAMP/PKA and insulin pathways
- Genes and neurons that regulate cocaine-induced behaviors: dlmo and the PDF-producing lateral neurons