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1. Introduction
2. Why drugs?
3. Why Drosophila?
4. Ethanol - a non specific drug
5. A quote by M. Ashburner
6. Models of addiction
7. The approach
8. What do ethanol-exposed flies do?
9. A really drunk fly
10. The inebriometer
11. Identification of important mutants and genes
12. The genetic screen process (1)
13. Cheapdate flies are sensitive in the inebriometer
14. Absorption and metabolism of cheapdate flies
15. Cheapdate is a mutation in amnesiac
16. Amnesiac rescues cheapdate's ethanol sensitivity
17. Drosophila mutants in cAMP signal transduction
18. The unbearable likeness of beings
19. Targeting NS disruptions
20. Regulation of ethanol snsitivity by PKA activity
21. GAL4/UAS system
22. Genetic manipulation of PKA activity
23. Reduced activity with PKA inhibitor expression
24. Acute PKA inhibition increases ethanol sensitivity
25. Use of a collection of GAL4 lines
26. P[GAL4] x UAS-lacZ
27. Brain expression of P[GAL4], line 201Y (1)
28. MB ablation does not alter ethanol sensitivity
29. Brain expression of P[GAL4], line 201Y (2)
30. PI cells express insulin-like peptides (dILPs)
31. Effect of PKA inhibition in IPCs
32. PI neurons project to the ring gland
33. The insulin receptor (INR) signaling pathway
34. Reduced dINR function increases EtOH sensitivity
35. Role of INR pathway in ethanol sensitivity
36. PI3K inhibition increases EtOH sensitivity
37. Expression of constituitivley active hFOXO
38. The insulin receptor (INR) signaling pathway
39. Insulin pathway and ethanol's rewarding effects
40. Mechanisms in fly's responses to cocaine
41. Psychostimulant exposure and behavioral analysis
42. Mock exposure
43. Acute cocaine exposure
44. A simple behavioral assay
45. Cocaine dose-response
46. Mechanism of cocaine action
47. Effect of dopamine depletion
48. The genetic screen process (2)
49. Effect of dLmo mutations on cocaine sensitivity
50. Lmo genomic region
51. How does dLMO work?
52. Where does dLmo function?
53. GAL4 expression in varios CNS patterns
54. Mapping the dLmo site of action
55. dLmo-GAL4 expression in PDF-positive cells
56. What is known about LNvs?
57. Does dLmo regulate cocaine sensitivity?
58. Two possible models for dLmo and LNvs function
59. Role of LNvs in cocaine sensitivity
60. The Working model
61. Summary
62. The Drosophila model and human drug addiction
63. Thank you

Topics Covered

• Behaviors induced by ethanol and cocaine in Drosophila
• Genes and pathways that regulate ethanol-induced behaviors: amnesiac, and the cAMP/PKA and insulin pathways
• Genes and neurons that regulate cocaine-induced behaviors: dlmo and the PDF-producing lateral neurons

Links

Series:

• The Legacy of Drosophila Genetics

Categories:

• Genetics & Epigenetics
• Neuroscience

Therapeutic Areas:

• Neurology

Talk Citation

Publication History

• Published on September 29, 2008
• Reviewed on September 25, 2017

Financial Disclosures

• Prof. Ulrike Heberlein has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.