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Monitoring presynaptic function with multiplexed imaging

Published on September 28, 2023   35 min

A selection of talks on Physiology & Anatomy

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0:00
Our lecture is about monitoring synoptic function using multiplex imaging.
0:09
Our aim is to try and understand basic synaptic mechanisms that underpin brain function. We know that memory formation in the brain involves changes in the strength of synaptic connections. These changes normally occur either through an altered postsynaptic receptor current, i.e. postsynaptic signal, or altered synaptic fidelity, neurotransmitter release probability or both. There is a growing evidence that
0:44
variations in neurotransmitter release probability are key for neural coding. This is because there are an enormous repertoire of advantages of having this release probability operator, if you like. First of it's got digital outcome, yes or no. It could display a long term, steady-state plasticity, it displays use and time dependent short term plasticity, it undergoes network dependent presynaptic control, but also all the above. You can see the theoretical background for these statements in that paper.
1:25
Neurotransmitter release is controlled by presynaptic calcium entry, and our aim is therefore to monitor both neurotransmitter release and presynaptic calcium dynamics simultaneously. This could be achieved using advanced methods of fluorescence microscopy.

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Monitoring presynaptic function with multiplexed imaging

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