• Introduction
• 63 min
  1. Drosophila genetics - the first 25 years

  • Prof. Dan Lindsley
• Establishment of the Primary Body Axes
• 28 min
  2. Homeotic genes in Drosophila's bithorax complex - The legacy of Ed Lewis

  • Prof. Francois Karch
• 30 min
  3. Limb development: how Drosophila genetics can be used to understand pattern formation

  • Prof. Konrad Basler
• Cell Type Specification and Organ Systems
• 63 min
  4. From germ cell specification to gonad formation

  • Prof. Ruth Lehmann
• 37 min
  5. Drosophila stem cells

  • Prof. Michael Buszczak
• 46 min
  6. Legacy of drosophila genetics: female germline stem cells

  • Prof. Michael Buszczak
• 34 min
  7. Intestinal stem cell-mediated repair in Drosophila 1

  • Prof. Tony Ip
• 30 min
  8. Intestinal stem cell-mediated repair in Drosophila 2

  • Prof. Tony Ip
• 43 min
  9. The miracle of morphogenesis, cell adhesion, polarity and cytoskeletal regulation

  • Prof. Mark Peifer
• 34 min
  10. Axon guidance in Drosophila

  • Prof. John Thomas
• 35 min
  11. Development and physiology of the heart

  • Prof. Rolf Bodmer
• 37 min
  12. Identification of host defenses in the Drosophila gut using genome-scale RNAi

  • Prof. Dominique Ferrandon
• Genome Organization and Function
• 53 min
  13. The genetic analysis of meiosis in Drosophila melanogaster females

  • Prof. R. Scott Hawley
• 46 min
  14. Genome scale analysis of cellular processes using RNAi

  • Dr. Amy Kiger
• 36 min
  15. Dorsal-ventral patterning of the Drosophila embryo

  • Prof. Mike Levine
• 34 min
  16. Bone morphogenetic protein (BMP) signaling in drosophila: from germline to early embryogenesis

  • Dr. Helena Araujo
• 27 min
  17. Genome-wide pooled CRISPR screen in arthropod cells

  • Prof. Norbert Perrimon
• Behavior
• 40 min
  18. The development of the peripheral nervous system in the fruitfly Drosophila

  • Prof. Hugo Bellen
• 54 min
  19. Genetics of chemosensory transduction: taste and smell

  • Dr. Leslie Vosshall
• 72 min
  20. Cracking the case of circadian rhythms by Drosophila genetics

  • Prof. Jeffrey C. Hall
• 46 min
  21. Sleep in Drosophila

  • Dr. Ralph Greenspan
• 91 min
  22. Drosophila's contribution to the genetics of learning and memory

  • Prof. Tim Tully
• 34 min
  23. Drosophila as a model for drug addiction

  • Prof. Ulrike Heberlein
• Mechanism of Human Disease
• 65 min
  24. Cross-genomic analysis of human disease genes

  • Prof. Ethan Bier
• 56 min
  25. Human neurodegenerative disease: insights from Drosophila genetics

  • Prof. Nancy Bonini
• 21 min
  26. Metastasis of Drosophila tumors

  • Prof. Allen Shearn
• 
  27. Rac-enhanced CAR immunotherapy: RaceCAR

  • Prof. Denise Montell
• 34 min
  28. Control of tissue growth: elucidation of the Hippo signaling pathway

  • Dr. Duojia Pan
• Evolution of Adaptive Novelties
• 56 min
  29. The evolution of morphological novelty

  • Prof. Nipam Patel
• 36 min
  30. The genetic architecture of complex traits: lessons from Drosophila

  • Prof. Trudy Mackay
• Archived Lectures *These may not cover the latest advances in the field
• 25 min
  31. Using gene expression information to provide insights into patterning and differentiation

  • Prof. Angelike Stathopoulos
• 49 min
  32. Regulation of gastrulation in Drosophila

  • Prof. Dr. Maria Leptin
• 31 min
  33. microRNA function in stem cells

  • Prof. Hannele Ruohola-Baker

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Circadian rhythms in different organisms
3. Circadian rhythms in period mutants
4. R.J Konopka
5. Circadian rhythms in timeless mutants
6. Phenotype of tim-blind mutants
7. Locomotor behavior in clock / cyc mutants
8. Andante mutants
9. The per-s mutantion is semi-dominant
10. doubletime mutants
11. shaggy gene
12. Isolation of per gene
13. Rescue of per-0 induced arrhythmicity
14. Expression of per gene
15. Protein product of per gene
16. Tissue staining with anti-per protein Antibodies (1)
17. Tissue staining with anti-per protein Antibodies (1)
18. Temporal expression pattern of per gene
19. PER cycling in the adult head
20. per mutations affect cycling of per expression
21. Alternating rhythms of per RNA and protein
22. Period and Timeless molecular and cellular cycles
23. How the clock works
24. cycle protein product
25. timekeeper mutants
26. Casein kinase 2 (CK2)
27. Clock kinases galore
28. A simplified model of a circadian system
29. Circadian elements: fly vs. mouse
30. Interlocked feedback loops
31. Results of constitutive expression of per and tim
32. TIM cycles in flies constitutively expressing tim
33. Examination of per and tim RNA cycling
34. per and tim doubly constitutive (1)
35. per and tim doubly constitutive (2)
36. per transgene missing the regulatory region
37. mRNA of the truncated-per transgene cycles
38. mRNA cycles of regulatory region fusions
39. Which subset regulate rhythmic behavior?
40. Expression pattern of "promotor-less" per DNA
41. disco1 mutant
42. disco1 arrhythmicity
43. Periodic adult emergence in disco1 mutants
44. Gal4\UAS expression system
45. Expression of PER\TIM in brain neurons
46. PER cells in the adults head
47. period is expressed throughout the fly
48. Pleiotropy of clock expression in mouse
49. timeless is also expressed throughout the fly
50. Expression of clock genes in Malpighian tubules
51. Tissues cycle throughout the Drosophila
52. per-luciferase expression system
53. Body segments show rhythmic bioluminescence
54. tim-luc body-part cycling
55. luc-reported per rhythm in isolated antenna
56. Antennal rhythm of olfactory sensitivity
57. How about (environmental) inputs to the clock?
58. Daily reset and seasonal re-adjustment of rhythms
59. Light absorption without going through the eye
60. Factors absorbing light
61. Rhythms produced from per-luc transgenic
62. Screening for novel rhythm-related mutants
63. Drosophila cryptochrome and a mutant form of it
64. Knocking-out Drosophila’s cry gene
65. norpA mutants: external photoreceptors blind
66. The light input system for circadian clock re-seting
67. per-luc cycling in cultured wings of cry-null flies
68. per-luc antennal rhythmicity in cry mutants (1)
69. per-luc antennal rhythmicity in cry mutants (2)
70. cry is required for EAG rhythms
71. Cryptochrome as an input factor
72. JETLAG and Light-induced TIMELESS degradation
73. Light-responsiveness defect in jetlag mutants
74. Light-responsiveness defect in LS-timeless
75. timeless alternative translation
76. Latitudinal cline of tim-AT frequency
77. Varying Thr-Gly length classes in the per gene
78. per cline
79. per variants and temperature compensation
80. Interspecific period variants
81. Phenotypes of period variants
82. Drosophila mating rhythms (1)
83. Drosophila mating rhythms (2)
84. Courtship behavior of Drosophila male
85. Features of courtship songs
86. Interpulse interval (IPI)
87. per mutations alter the (short-term) song cycle
88. Related species have different cycles and IPIs
89. Song rhythm difference between two species
90. Interspecific per transformation
91. How about outputs from the clock?
92. PER as regulator of genes in output pathways
93. Output rhythms
94. Drosophila pigment dispersing factor (PDF)
95. Co-expression of per and pdf in the brain
96. Circadian locomotor rhythms of the pdf-null mutant
97. pdf-null mutant behavior in constant darkness
98. pdf expression patterns in clock-jrk mutant
99. Use if Gal4-UAS system to study pdf gene
100. Targeted cell-ablation and rhythms
101. The luc sniffer enhancer-trap constract
102. luc-assisted genetic analysis of the clock (1)
103. luc-assisted genetic analysis of the clock (2)
104. Identifying novel Drosophila clock controlled genes
105. Sleep-wake cycles in Drosophila
106. Reduced sleep in shaker mutants
107. Expression of neuropeptide F gene (1)
108. Expression of neuropeptide F gene (2)
109. Use if Gal4-UAS system to study npf gene
110. Phenotype of npf-gal4 transgene
111. Summary
112. Rhythm-related genetic loci in D. melanogaster

Topics Covered

• Rhythm mutants
• Identification of the corresponding genes and inferences about their products
• Manipulation of such genes amd product functions to elucidate not only the neural substrates of behavioral rhythmicities, but also the intracellular mechanisms that comprise biological clocks
• Identification of additional rhythm-related genes in their normal forms, followed by analogous manipulations of them
• Analyses of core clock functions in context of rhythm-related processes that surround such central "pacemaking", that is: environmental inputs to the clock and how its actions are output to regulate revealed rhythms

Links

Series:

• The Legacy of Drosophila Genetics

Categories:

• Genetics & Epigenetics

Talk Citation

Publication History

• Published on September 29, 2008
• Reviewed on May 31, 2018

Financial Disclosures

• Prof. Jeffrey C. Hall has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.