Registration for a live webinar on 'Innovative Vaccines and Viral Pathogenesis: Insights from Recent Monkeypox (Mpox) Research' is now open.
See webinar detailsWe noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
Printable Handouts
Navigable Slide Index
- Introduction
- Circadian rhythms in different organisms
- Circadian rhythms in period mutants
- R.J Konopka
- Circadian rhythms in timeless mutants
- Phenotype of tim-blind mutants
- Locomotor behavior in clock / cyc mutants
- Andante mutants
- The per-s mutantion is semi-dominant
- doubletime mutants
- shaggy gene
- Isolation of per gene
- Rescue of per-0 induced arrhythmicity
- Expression of per gene
- Protein product of per gene
- Tissue staining with anti-per protein Antibodies (1)
- Tissue staining with anti-per protein Antibodies (1)
- Temporal expression pattern of per gene
- PER cycling in the adult head
- per mutations affect cycling of per expression
- Alternating rhythms of per RNA and protein
- Period and Timeless molecular and cellular cycles
- How the clock works
- cycle protein product
- timekeeper mutants
- Casein kinase 2 (CK2)
- Clock kinases galore
- A simplified model of a circadian system
- Circadian elements: fly vs. mouse
- Interlocked feedback loops
- Results of constitutive expression of per and tim
- TIM cycles in flies constitutively expressing tim
- Examination of per and tim RNA cycling
- per and tim doubly constitutive (1)
- per and tim doubly constitutive (2)
- per transgene missing the regulatory region
- mRNA of the truncated-per transgene cycles
- mRNA cycles of regulatory region fusions
- Which subset regulate rhythmic behavior?
- Expression pattern of "promotor-less" per DNA
- disco1 mutant
- disco1 arrhythmicity
- Periodic adult emergence in disco1 mutants
- Gal4\UAS expression system
- Expression of PER\TIM in brain neurons
- PER cells in the adults head
- period is expressed throughout the fly
- Pleiotropy of clock expression in mouse
- timeless is also expressed throughout the fly
- Expression of clock genes in Malpighian tubules
- Tissues cycle throughout the Drosophila
- per-luciferase expression system
- Body segments show rhythmic bioluminescence
- tim-luc body-part cycling
- luc-reported per rhythm in isolated antenna
- Antennal rhythm of olfactory sensitivity
- How about (environmental) inputs to the clock?
- Daily reset and seasonal re-adjustment of rhythms
- Light absorption without going through the eye
- Factors absorbing light
- Rhythms produced from per-luc transgenic
- Screening for novel rhythm-related mutants
- Drosophila cryptochrome and a mutant form of it
- Knocking-out Drosophila’s cry gene
- norpA mutants: external photoreceptors blind
- The light input system for circadian clock re-seting
- per-luc cycling in cultured wings of cry-null flies
- per-luc antennal rhythmicity in cry mutants (1)
- per-luc antennal rhythmicity in cry mutants (2)
- cry is required for EAG rhythms
- Cryptochrome as an input factor
- JETLAG and Light-induced TIMELESS degradation
- Light-responsiveness defect in jetlag mutants
- Light-responsiveness defect in LS-timeless
- timeless alternative translation
- Latitudinal cline of tim-AT frequency
- Varying Thr-Gly length classes in the per gene
- per cline
- per variants and temperature compensation
- Interspecific period variants
- Phenotypes of period variants
- Drosophila mating rhythms (1)
- Drosophila mating rhythms (2)
- Courtship behavior of Drosophila male
- Features of courtship songs
- Interpulse interval (IPI)
- per mutations alter the (short-term) song cycle
- Related species have different cycles and IPIs
- Song rhythm difference between two species
- Interspecific per transformation
- How about outputs from the clock?
- PER as regulator of genes in output pathways
- Output rhythms
- Drosophila pigment dispersing factor (PDF)
- Co-expression of per and pdf in the brain
- Circadian locomotor rhythms of the pdf-null mutant
- pdf-null mutant behavior in constant darkness
- pdf expression patterns in clock-jrk mutant
- Use if Gal4-UAS system to study pdf gene
- Targeted cell-ablation and rhythms
- The luc sniffer enhancer-trap constract
- luc-assisted genetic analysis of the clock (1)
- luc-assisted genetic analysis of the clock (2)
- Identifying novel Drosophila clock controlled genes
- Sleep-wake cycles in Drosophila
- Reduced sleep in shaker mutants
- Expression of neuropeptide F gene (1)
- Expression of neuropeptide F gene (2)
- Use if Gal4-UAS system to study npf gene
- Phenotype of npf-gal4 transgene
- Summary
- Rhythm-related genetic loci in D. melanogaster
Topics Covered
- Rhythm mutants
- Identification of the corresponding genes and inferences about their products
- Manipulation of such genes amd product functions to elucidate not only the neural substrates of behavioral rhythmicities, but also the intracellular mechanisms that comprise biological clocks
- Identification of additional rhythm-related genes in their normal forms, followed by analogous manipulations of them
- Analyses of core clock functions in context of rhythm-related processes that surround such central "pacemaking", that is: environmental inputs to the clock and how its actions are output to regulate revealed rhythms
Talk Citation
Hall, J.C. (2018, May 31). Cracking the case of circadian rhythms by Drosophila genetics [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/WIDU5390.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Jeffrey C. Hall has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
A selection of talks on Genetics & Epigenetics
Transcript
Please wait while the transcript is being prepared...
0:00
This presentation will be about biological
rhythms, and most of the rhythmic
characteristics, that I'll be speaking
about have to do with daily rhythms.
0:11
Including those,
that are exhibited by a fruit fly,
one of which is actually indicated
over there on the left, Drosophila.
The display here of a variety of
different kinds of daily rhythms,
is not done completely errantly.
Because several features, of the outcomes
of studying rhythms Drosophila,
were better than the investigators
intended in a way.
Because they spoke to certain
components or even several ones,
of rhythm control that are operating in
many other different kinds of organisms,
which is one of the pleasant surprises
involving studies of rhythmicity in
the fruit fly, Drosophila.
I and colleagues began,
looking into rhythms Drosophila
back in the late 1970s or so.
And this refers in a way to the fact that
this treatment of rhythms in Drosophila,
will have two attributes, one, that
it's gonna be largely based on genetics,
genetic variance, either mutants or
genetic variants that are made by
various molecular genetic manipulations.
Second, I'm largely going to be
treating this subject historically,
I will be flashing through many of
the pictures here without delving
all that deeply into the chronobiological
matter in question, but
mostly trying to give a survey of how
a genetic approach to studying rhythms,
notably daily ones,
how the genetic approach to these arguably
interesting biological problems,
came out very well and
revealed much in the way about,
how rhythms of this sort are regulated.
Not only in Drosophila as previewed,
but also in other organisms.