• Introduction
• 30 min
  1. Prions and amyloids: introduction

  • Prof. Reed Wickner
• Mammalian Prions
• 26 min
  2. Transgenic mouse models of prion diseases

  • Prof. Glenn Telling
• 31 min
  3. Mechanism of prion generation in vitro

  • Dr. Surachai Supattapone
• Non-Prion Amyloids
• 48 min
  4. Similarities between prion diseases and other neurodegenerative diseases

  • Prof. John Hardy
• 43 min
  5. Molecular structure of amyloid and prion fibrils: insights from solid state NMR

  • Dr. Robert Tycko
• Yeast Prions
• 42 min
  6. Chaperones and prions

  • Prof. Yury Chernoff
• Beneficial Amyloids
• 52 min
  7. The dark side of amyloid: PMEL, a natural amyloid in melanosome biogenesis

  • Prof. Michael Marks
• Archived Lectures *These may not cover the latest advances in the field
• 41 min
  8. Predicting TSE transmission

  • Prof. Jean Manson
• 53 min
  9. Generation and propagation of infectious prions by cyclic amplification of protein misfolding

  • Prof. Claudio Soto
• 30 min
  10. Yeast and fungal prions: a help or a hindrance?

  • Prof. Reed Wickner
• 42 min
  11. [PIN+]: prions beget prions

  • Prof. Susan Liebman
• 36 min
  12. Yeast prions and protein chaperones

  • Dr. Daniel Masison
• 54 min
  13. Mechanisms of yeast prion propagation

  • Prof. Mick Tuite
• 31 min
  14. Propagation and variability of the yeast [PSI+] prion

  • Prof. Michael Ter-Avanesyan
• 43 min
  15. The genetics and biology of the [Het-s] prion of Podospora

  • Prof. Sven Saupe
• 33 min
  16. The structure of amyloid fibrils of the HET-s (218-289) prion as seen by solid-state NMR

  • Prof. Beat Meier

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Prions
3. Prions are infectious amyloids
4. Amyloid fibres growth process
5. Fragmentation of prion polymers
6. Important questions concerning prions
7. In vivo approach - prion propagation mechanism
8. Yeast prion propagation and rapid cell division
9. The PSI+ prion and its associated phenotype
10. The prion protein Sup35p: eRF3 (1)
11. Yeast prions are efficiently propagated
12. Prion seeds and the 'propagon'
13. The molecular chaperone Hsp104 discovery
14. The molecular chaperone Hsp104 discovery
15. Propagation of PSI+ prion and the role of Hsp104
16. Hsp104 activity Inhibition leads to propagons loss
17. Guanidinium ion is an Hsp104-specific inhibitor
18. GdnHCl eliminates [PSI+] prion from growing cells
19. GdnHCl-induced [PSI+] curing and cell division (1)
20. GdnHCl-induced [PSI+] curing and cell division (2)
21. Calculating propagon numbers
22. Proportion of propagons passed to daughter (1)
23. Proportion of propagons passed to daughter (2)
24. The advanced stochastic model
25. Mechanism of new propagon generation
26. The generation of new propagons
27. Hsp104 elevated levels block [PSI+] propagation
28. Isolation and genetic behaviour of PNM mutants
29. PNM1 alleles of HSP104 show a defect in function
30. The prion protein Sup35p: eRF3 (2)
31. The prion protein Sup35p: eRF3 (3)
32. PNM2-1 encodes a Sup35p defective form
33. The effect of PNM2-1 mutation on propagation
34. Sup35p primary sequence and [PSI+] propagation
35. Other amino acid substitutions at Gly58
36. PNM2-1 mutation reduces PFD flexibility
37. An overview of [PSI+] prion propagation
38. Summary
39. Acknowledgements
40. References

Topics Covered

• Prions as infectious amyloids
• Yeast [PSI+] prion and Sup35p
• Molecular chaperone Hsp104
• Guanidine hydrochloride as a prion propagation inhibitor
• Propagons
• Estimating propagon numbers using a stochastic model
• Generation of new propagons
• Propagation-defective mutants (PNM)
• Role of primary sequence information
• Yeast prion propagation: the unanswered questions

Links

Series:

• Prions and Amyloids

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on September 4, 2008
• Archived on February 25, 2021

Financial Disclosures

• Prof. Mick Tuite has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.