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1. Introduction
2. Protein misfolding is a significant clinical problem
3. Heat shock proteins protect cells from stress
4. Cellular processes involving Hsp70
5. Regulated reaction cycle controls Hsp70 function
6. Yeast prions
7. Sup35 solubility determines suppressor phenotype
8. Chaperone mutations impair amyloid propagation
9. Chaperones activity and prions "seed" replication
10. L483W mutation of Hsp70
11. Location of mutations on Hsp70 structure
12. Altered substrate binding and amyloid propagation
13. Fes1 affects [PSI+] as anticipated
14. Hsp70 co-chaperones and amyloid propagation
15. Sup35 prion domain-GFP fusion protein
16. Aggregates of Sup35 are larger in Hsp70 mutant
17. Larger polymers or larger aggregates of polymers?
18. Size of Sup35 polymers in Hsp70 mutant
19. Activity of mutant Hsp70
20. Cytosolic Hsp70 Ssa isoforms
21. Hsp70 isoforms influence prions differently
22. Differences between Ssa1 and Ssa2
23. Hsp104 resolubilizes proteins from aggregates
24. Guandine-hydrochloride "cures" yeast of prions
25. Kinetics of guanidine curing of [PSI+]
26. Hsp104 fragments prion polymers
27. Hsp104 makes new prion "seeds"
28. Threading activity correlates with [PSI+] strength
29. Amyloid breaking by Hsp104/70/40 machinery
30. [PSI+] prion requiring modestly elevated Hsp104
31. Effects of elevated Hsp104 on [PSI+]
32. Ssa1 antagonizes curing of [PSI+] by Hsp104
33. NTD required for excess Hsp104 to cure [PSI+]
34. Effects of altered chaperone expression on prions
35. Chaperone involvement in prion replication

Topics Covered

• Role of protein chaperones in protein folding
• Yeast prions as amyloid forms of cellular proteins
• Requirement of chaperone machinery for prion propagation
• Chaperone mutants adversely affect prions and provide insight into chaperone/amyloid interactions

Links

Series:

• Prions and Amyloids

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on September 4, 2008
• Archived on February 25, 2021

Financial Disclosures

• Dr. Daniel Masison has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.