• Bioorganic Chemistry and the Origins of Chemical Biology
• 56 min
  1. Biomimetic catalysis

  • Prof. Ronald Breslow
• 50 min
  2. Bioorganic studies of vision

  • Prof. Koji Nakanishi
• 48 min
  3. Perspectives on biological catalysis

  • Prof. Stephen Benkovic
• Nucleic Acids and Drug/Nucleic Acid Interactions
• 41 min
  4. The importance of nucleotide size in the chemistry and biology of DNA

  • Prof. Eric Kool
• 27 min
  5. Site-specifically modified ribosomal RNAs

  • Prof. Christine Chow
• 26 min
  6. Creation of tools for the investigation of oxidative damage to nucleic acids

  • Dr. Mel Bedi
• 48 min
  7. Drug-DNA interactions of the mitomycins

  • Prof. Maria Tomasz
• Protein Function and Cellular Signaling
• 41 min
  8. Chemical synthesis of proteins

  • Prof. Lei Liu
• 55 min
  9. Visualizing and manipulating phosphoinositide and phospholipid signaling

  • Prof. Glenn Prestwich
• 86 min
  10. Phosphoserine/threonine binding domains: molecular integrators of protein kinase signaling in cell cycle control and cancer

  • Dr. Michael Yaffe
• 59 min
  11. Polydactyl zinc finger proteins: software and hardware for genomes

  • Prof. Carlos Barbas
• Peptides, Small-Molecules and Chemical Diversity
• 41 min
  12. Chemical synthesis of peptides and peptide libraries

  • Prof. Victor Hruby
• Proteomics: The Study of Biomolecules and Interactions
• 46 min
  13. Quantitative proteomics

  • Prof. Ruedi Aebersold
• Archived Lectures *These may not cover the latest advances in the field
• 51 min
  14. Chemical protein synthesis: total synthesis of proteins for biological research

  • Prof. Stephen Kent
• 57 min
  15. Solid phase peptide synthesis

  • Prof. Stephen Kent
• 39 min
  16. Mapping small molecule binding sites with phage display technology

  • Dr. Lee Makowski
• 32 min
  17. Tagged library for chemical genetics

  • Dr. Young-Tae Chang
• 30 min
  18. Covalently constrained alpha-helices

  • Dr. Paramjit Arora
• 34 min
  19. SICLOPPS: genetic system for production of backbone cyclized peptides

  • Dr. Sergey Savinov
• 35 min
  20. Probing protein-protein interactions with peptide libraries and arrays

  • Dr. Zhou Songyang

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. From sequences to functions
3. Signaling via protein tyrosine kinases
4. Protein modules in signal transduction
5. Application of peptide library approach
6. Oriented peptide library
7. rasGAP C-terminal SH2 domain
8. Area for improvement
9. OPAL: oriented peptide array library
10. AutoSpot for peptide arrays
11. Amino acid scan
12. OPAL screen protocol
13. MPM2 antibody
14. MPM2 antibody preference
15. OPAL analyses of SH2 domains (1)
16. OPAL analyses of SH2 domains (2)
17. SH2 domains of SHP2
18. Preference of SHP2 SH2 domain
19. OPAL protocol for protein kinases
20. OPAL analyses of protein kinase A
21. Phosphorylation of substrate peptides by PKA
22. OPAL analyses of protein kinases
23. Transformation of array data
24. Database prediction of kinase substrates
25. Summary
26. Acknowledgements

Topics Covered

• Protein-protein interactions as key to understanding signaling pathways
• Signaling through tyrosine phosphorylation
• Using peptide libraries to study specificities of protein modules
• Peptide library arrays (OPAL)
• OPAL strategies, examples and advantages

Links

Series:

• Chemical Biology

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on August 14, 2008
• Archived on February 25, 2021

Financial Disclosures

• Dr. Zhou Songyang has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.