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Navigable Slide Index

1. Introduction
2. From sequences to functions
3. Signaling via protein tyrosine kinases
4. Protein modules in signal transduction
5. Application of peptide library approach
6. Oriented peptide library
7. rasGAP C-terminal SH2 domain
8. Area for improvement
9. OPAL: oriented peptide array library
10. AutoSpot for peptide arrays
11. Amino acid scan
12. OPAL screen protocol
13. MPM2 antibody
14. MPM2 antibody preference
15. OPAL analyses of SH2 domains (1)
16. OPAL analyses of SH2 domains (2)
17. SH2 domains of SHP2
18. Preference of SHP2 SH2 domain
19. OPAL protocol for protein kinases
20. OPAL analyses of protein kinase A
21. Phosphorylation of substrate peptides by PKA
22. OPAL analyses of protein kinases
23. Transformation of array data
24. Database prediction of kinase substrates
25. Summary
26. Acknowledgements

Topics Covered

• Protein-protein interactions as key to understanding signaling pathways
• Signaling through tyrosine phosphorylation
• Using peptide libraries to study specificities of protein modules
• Peptide library arrays (OPAL)
• OPAL strategies, examples and advantages

Links

Series:

• Chemical Biology

Categories:

• Biochemistry

Talk Citation

Songyang, Z. (2008, August 14). Probing protein-protein interactions with peptide libraries and arrays [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 22, 2024, from https://doi.org/10.69645/DNDT9444.
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Publication History

• Published on August 14, 2008
• Archived on February 25, 2021

Financial Disclosures

• Dr. Zhou Songyang has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.