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Navigable Slide Index

1. Introduction
2. Challenges in the post-genomic era
3. Functional assignments through perturbation
4. Small-molecule antagonism of protein complexes
5. Extraordinary solutions for a nontrivial problem
6. Systematic search for protein modulators (1)
7. Bifunctional and mini-inteins
8. Chemistry of intein splicing
9. Cyclizative ligation: the concept
10. SICLOPPs
11. Peptides as biological probes
12. Cyclic vs. linear
13. Intracellular production
14. Recombinant cyclic peptides
15. Implementation of the concept
16. Affinity chromatography
17. SDS-PAGE analysis
18. Cyclic peptide detection
19. Intracellular production of bioactive peptide
20. Library construction
21. Scope and limitations
22. Epitope 'grafting' for templated splicing
23. Streptavidin-binding peptide
24. Binding strength
25. Linking function to survival
26. Systematic search for protein modulators (2)
27. Conditional transcription system (1)
28. Conditional transcription system (2)
29. Systematic search for protein modulators (3)
30. Ribonucleotide reductase: dissociative inhibition
31. Searching for dissociative inhibitors
32. Dissociative inhibitors?
33. Protein-protein dissociation assay (1)
34. Protein-protein dissociation assay (2)
35. Peptide display ELISA
36. Conclusions
37. Acknowledgements

Topics Covered

• SICLOPPS: method for production of genetically encoded backbone cyclized peptides
• Cyclic peptides as probes of biological function
• Splicing of a circularly rearranged split-intein
• Production and isolation of backbone cyclic peptides
• Generation of combinatorial libraries
• Selection of protein-protein inhibitors using a bacterial two-hybrid assay
• Biochemical characterization of dissociative inhibitors

Links

Series:

• Chemical Biology

Categories:

• Biochemistry
• Methods

Talk Citation

Savinov, S. (2008, August 14). SICLOPPS: genetic system for production of backbone cyclized peptides [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/VBQH7562.
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Publication History

• Published on August 14, 2008
• Archived on February 25, 2021

Financial Disclosures

• Dr. Sergey Savinov has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.