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1. Introduction
2. Chemical protein synthesis
3. Emil Fischer- Nobel prize in chemistry (1902)
4. HIV-1 protease
5. Electronic properties of the catalytic apparatus
6. Defining the 'Aspartyl proteases'
7. Case study: 'backbone engineering' HIV-1 PR
8. Dissecting the energetics of C-cap termination
9. The alpha-helix common structural motif
10. How does a protein alpha-helix terminate?
11. Case study: Ubiquitin (76 aa)
12. Conformation vs. solvation effects
13. Separting of solvation from conformation effects
14. Seven synthetic ubiquitins
15. X-ray crystal structure of a protein diastereomer
16. Global and local structures are highly conserved
17. Stability measurements of [Aaa35] Ubiquitins
18. Energetic contributions at helix C' position
19. Erythropoietin (Epo)- stimulates hematopoiesis
20. Recombinant Epo
21. Case study: synthetic erythropoiesis protein
22. Synthetic 'glycan mimetic'
23. Synthetic scheme
24. SEP - analytical data
25. SEP - features
26. SEP - biological properties
27. Glycoprotein synthesis
28. Molecular basis of protein function
29. The synthetic challenge
30. Classical solution synthesis
31. Stepwise solid phase synthesis
32. Conventional approaches 'stalled out'
33. Chemical protein synthesis - re-examined
34. Unprotected peptides - a challenge
35. Chemical protein synthesis - chemical ligaion
36. Principles of chemical ligation
37. Thioester- forming ligation
38. Total synthesis of D- & L-HIV-1 protease
39. Crystal structure of D-HIV-1 protease
40. Native chemical ligation (1)
41. Thioester-linked ligation product
42. Native chemical ligation (2)
43. Case study: Crambin (46 aa; 6 Cys)
44. Crambin
45. Total chemical synthesis of Crambin
46. A 'One-Pot' total chemical synthesis of Crambin
47. Characterization of synthetic Crambin
48. Crambin synthesis - overall yield
49. Ligation of unprotected peptides
50. Chemical protein synthesis - to date
51. Impact of chemical ligation
52. CPS current challenges
53. Better catalyst for native chemical ligation
54. CPS of HIV-1 PR
55. Current challenges (1)
56. "Middle" segments
57. Kinetically-controlled ligation
58. Crambin: convergent synthesis
59. Crambin synthesis- analytical data
60. Targets to which convergent CPS is being applied
61. Current challenges (2)
62. Solid phase chemical ligation of EETI-II (1)
63. Solid phase chemical ligation of EETI-II (2)
64. Solid phase chemical ligation of EETI-II (3)
65. Folding synthetic proteins
66. In vitro folding of synthetic proteins
67. AOP-RANTES - folding
68. AOP-RANTES- conformational homogeneity
69. Crystal structure of AOP-RANTES
70. Human secretory PLA4
71. CPS - what is the "killer app"?
72. 'Single molecule' enzymology
73. Entree to a protein world
74. Acknowledgements

Topics Covered

• HIV-1 protease
• Electronic properties of the catalytic apparatus
• Defining the 'aspartyl proteases'
• 'Backbone engineering' HIV-1 PR
• The alpha-helix
• Ubiquitin (76 aa)
• Conformation vs. solvation effects
• Global and local structures are highly conserved
• Stability measurements of [Aaa35] ubiquitins
• Energetic contributions at helix C' position
• Erythropoietin (Epo)
• Synthetic erythropoiesis protein
• Synthetic 'glycan mimetic'
• Glycoprotein synthesis
• Molecular basis of protein function
• The synthetic challenge
• Classical solution synthesis
• Stepwise solid-phase synthesis
• Chemical protein synthesis
• Unprotected peptides
• Principles of chemical ligation
• Thioester-forming ligation
• Total synthesis of D- & L-HIV-1 protease
• Crystal structure of D-HIV-1 protease
• Native chemical ligation
• Crambin (46 aa; 6 Cys)
• CPS: current challenges

Links

Series:

• Chemical Biology

Categories:

• Biochemistry

Talk Citation

Kent, S. (2008, August 14). Chemical protein synthesis: total synthesis of proteins for biological research [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 19, 2025, from https://doi.org/10.69645/UXKO6902.
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Publication History

• Published on August 14, 2008
• Archived on August 13, 2020

Financial Disclosures

• Prof. Stephen Kent has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.