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Printable Handouts
Navigable Slide Index
- Introduction
- ER network
- ER function
- The ER quality control system
- Molecular chaperones and folding enzymes
- ER luminal proteins
- Calreticulin/calnexin cycle
- Calreticulin/calnexin substrate specificty
- ER-associated protein degradation
- Calreticulin, calnexin, ERp57: quality control cycle
- Model of calreticulin structure
- Model of calnexin structure
- Model of calnexin/calreticulin and ERp57 complex
- Thioloxidase and disulfide isomerase mechanism
- PDI family of proteins
- Protein folding and Ca homeostasis summary
- Protein folding diseases
- Calreticulin-deficient embryos
- Calreticulin promoter activity during cardiogenesis
- Myofibrillogenesis in the absence of calretculin
- Calreticulin vs. calnexin deficiency
- Calnexin-deficient mice
- Schematic view of ER role in calcium homoestasis
- Calcium binding chaperones of the ER lumen
- The role of CRT C-domain in calcium buffering
- Over expression of calrecticulin
- ER luminal free calcium and calreticulin
- Thapsigargin-induced calcium release
- Agonist-induced calcium release
- IP3-induced calcium release
- Bradykinin binding to the bradykinin receptor
- Agonist-induced Ca release and N+P domains
- CRT cells loss vs. gain of function
Topics Covered
- Quality control
- Chaperones and folding enzymes in the ER
- Prevention of the deployment of misfolded proteins
- Impaired protein folding in Alzheimer's disease, CMT and cystic fibrosis
- Deficiency in ER calcium-binding chaperones impacts embryonic development
- The lymphatic drainage system of the brain
- The gut-immune-brain axis
- Exercise, immunity, and mental health
- Gut microbiome
- Update talk: ER stress
- Update talk: The Unfolded Protein Response (UPR)
- Update talk: ER stress sensors
- Update talk: Inositol-Requiring Element 1 (IRE1α)
- Update talk: Stress-induced activation of the calreticulin gene
- Update talk: Targeting IRE1α to prevent cardiac fibrosis
- Update talk: TUDCA prevents cardiac fibrosis
Talk Citation
Michalak, M. (2022, January 30). The endoplasmic reticulum: protein folding and control of calcium homeostasis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 11, 2024, from https://doi.org/10.69645/VCCV1353.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Marek Michalak has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Update Available
The speaker addresses developments since the publication of the original talk. We recommend listening to the associated update as well as the lecture.
- Full lecture Duration: 32:00 min
- Update Duration: 8:21 min
The endoplasmic reticulum: protein folding and control of calcium homeostasis
A selection of talks on Biochemistry
Transcript
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0:05
The endoplasmic reticulum is an extensive membrane network of cisterna,
sac like structures held together by the cytoskeleton.
The phospholipid membrane encloses a space,
the luminal space from the cytosol.
The two varieties of ER are called rough endoplasmic reticulum,
and smooth endoplasmic reticulum.
The slide shows EM analysis of mouse
embryonic fibroblast and shows extended tubules of the rough ER.
The surface of the rough endoplasmic reticulum is studded with
protein manufacturing ribosomes giving it a rough appearance.
Ribosomes only bind to the ER once it
begins to synthesize a protein destined for sorting.
The membrane of the rough endoplasmic reticulum is
continuous with the outer layer of the nuclear envelope.
Although there is no continuous membrane between the rough ER and the Golgi apparatus.
Membrane bound vesicles are shuttle proteins between these two compartments.
The rough endoplasmic reticulum works in concert with the Golgi
complex to target new proteins to the appropriate destination.
Smooth endoplasmic reticulum functions in several metabolic processes,
including synthesis of lipids,
metabolism of carbohydrate, and calcium homeostasis.
It is connected to the nuclear envelope.
The smooth ER is best known for its storage of calcium ions in muscle cells.
1:30
The endoplasmic reticulum serves many general functions,
including the facilitation of protein folding and the transport of synthesized proteins.
Phospholipid synthesis, cholesterol and steroids synthesis,
detoxification reaction in control of many cellular signaling processes,
including calcium homeostasis, regulation of gene expression,
apoptosis, communication with mitochondria and the plasma membrane.
The ER membrane is one of
the major calcium storage organelles equipped with a number of
calcium transport proteins and
several calcium buffering proteins localized in the lumen.
ER luminal calcium concentration is relatively
high and could be as high as two to three millimolar,
where cytoplasmic calcium concentration resting
cells is approximately 50 to a 100 nanomole.
Calcium in the luminal of ER is bound to ER resident calcium buffering proteins.
Calcium is released from the ER by IP3R in response to hormonal stimulation of the cell.
Calcium released from the ER is sensed by STIM,
which transmits this information to plasma membrane calcium channels,
responsible for so-called store operated calcium channel.
Calcium is removed from the cytoplasm by SERCA endoplasmic sarcoplasmic calcium ATPase.
This talk will focus on two major functions of ER synthesis and folding of proteins,
and an ER role as a regulator of calcium homeostasis.
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