• Architecture of the ribosome RNA-protein machine assembly
• 49 min
  1. High resolution structure of ribosomal subunits RNA/RNA and protein/RNA interactions

  • Prof. Nenad Ban
• 38 min
  2. Assembly of the 30S ribosomal subunit in vitro and in cells

  • Prof. James Williamson
• Decoding and peptide bond formation
• 46 min
  3. How aminoacyl-tRNA synthetases translate the genetic code

  • Dr. Stephen Cusack
• 42 min
  4. The structural basis for how the large ribosomal subunit catalyses peptide bond formation

  • Prof. Thomas Steitz
• 47 min
  5. Elongation of protein synthesis: structural basis of the process of decoding

  • Prof. Marina Rodnina
• Initiation of protein synthesis
• 49 min
  6. The structure-based mechanism of translation initiation in bacteria and eukaryotes

  • Prof. Jeffrey Kieft
• 48 min
  7. Factor independent initiation of protein synthesis by IRES RNAs

  • Prof. Jeffrey Kieft
• Elongation and termination of protein synthesis
• 41 min
  8. Translocation: movement of tRNA and mRNA through the ribosome

  • Prof. Harry Noller
• 31 min
  9. Recoding: getting more out of the message by shifting reading frame and redefining codon meaning

  • Prof. John Atkins
  • Prof. Raymond Gesteland
• Co-translational protein secretion
• 75 min
  10. Mechanism of translocon function: current insights and models

  • Prof. Arnold Driessen
• How antibiotics target the ribosome
• 29 min
  11. Antibiotics that target the 50S ribosome subunit: resistance and other considerations

  • Dr. Joyce Sutcliffe
• 35 min
  12. Antibiotic inhibition of ribosome function

  • Dr. Daniel Wilson
• 45 min
  13. Structure-based drug design targeting infectious disease

  • Dr. Erin Duffy
• Archived Lectures *These may not cover the latest advances in the field
• 26 min
  14. Structural insights into aminoacyl-tRNA delivery by EF-Tu and translocation by EF-G

  • Prof. Joachim Frank
• 21 min
  15. Introduction to the ribosome

  • Prof. Anders Liljas
• 20 min
  16. The structure of the intact ribosome and ribosomal subunit interactions

  • Dr. Jamie H. Doudna Cate
• 42 min
  17. Structural insights into decoding of mRNA by the ribosome

  • Prof. Venki Ramakrishnan
• 63 min
  18. Chemistry of peptide bond formation

  • Prof. Rachel Green
• 49 min
  19. The interaction of antibiotics with the large ribosomal subunit from Haloarcula marismortui

  • Prof. Peter Moore

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Recoding
3. Recoding features
4. Codon redefinition
5. Bacterial selenocysteine encoding
6. Selenocysteine encoding: 50S subunit interface
7. Mammalian selenocysteine: selenoprotein N
8. TMV RNA
9. MuLV gag stop codon redefinition
10. StopGo in foot-and-mouth disease virus
11. Frameshifting
12. Release factor 2 frameshift: plentiful RF2
13. Release factor 2 frameshift: RF2 limiting
14. E-site spacing at initiation
15. Conservation of RF2 shift site
16. ORF architecture
17. Human antizyme (+1) frameshift
18. Antizyme in polyamine regulation
19. Two models for Ty3 (+1) frameshifting
20. dnaX frameshift - DNA Pol subunits
21. "dnaX" transcriptional slippage
22. Beet western yellows virus: pseudoknot
23. Retroviruses - why recoding?
24. HIV gag - pol frameshifting
25. A frameshift signal: 4 kb 3' (1)
26. A frameshift signal: 4 kb 3' (2)
27. Discontiguity
28. T4 gene 60 bypassing
29. Ribosomal protein L9
30. tmRNA trans-translation
31. tmRNA-ribosome complex
32. Summary

Topics Covered

• Dynamic reprogramming of genetic readout
• 3 broad types
• Change in linearity, i.e. programmed ribosomal frame shifting
• Codon redefinition, i.e. commonly "stop codons" specifying an amino acid, a standard one or selenocysteine
• Discontiguity

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Atkins, J. and Gesteland, R. (2008, May 15). Recoding: getting more out of the message by shifting reading frame and redefining codon meaning [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved March 20, 2025, from https://doi.org/10.69645/WZRW7329.
Export Citation (RIS)

Publication History

• Published on May 15, 2008

Financial Disclosures

• Prof. John Atkins has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
• Prof. Raymond Gesteland has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.